Руководство по эксплуатации спектрофотометра uv 1800

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  206-97040A Feb. 2008 INSTRUCTION MANUAL System User’s Guide UV-1800 SHIMADZU SPECTROPHOTOMETER Read the instruction manual thoroughly before you use the product. Keep this instruction manual for future reference.
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  Shimadzu representative. • To ensure safe operation, read the Safety Instructions before using the instrument. • To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, or re- installation (after the product is moved) is required.
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  Emphasizes additional information that is provided to ensure the proper use of this NOTE product. The symbol used in this manual is as follows: Indication of location for related information in the instruction manuals UV-1800 SERIES…
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  Otherwise, the instrument could tip over or fall off the table. • Avoid installation sites that are exposed to corrosive gases or excessive dust. These adverse conditions may be detrimental to maintaining instrument performance and may shorten its service life. UV-1800 SERIES…
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  Installation Precautions To ensure safe operation, contact your Shimadzu representative for installation, adjustment, or re-installation after moving the instrument to a different site. WARNING • Take measures to prevent the instrument from falling in the event of earthquake or other disaster.
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  The main cover does not need to be removed for routine maintenance, inspection and adjustment. Have your Shimadzu representative perform any repairs requiring removal of the main cover. • If the power cord plug gets dusty, remove the plug from the power outlet and wipe away the dust with a dry cloth.
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  In case of electrical failure, perform the following operations: 1. Press the » » side of the power switch located on the right side bottom of the UV-1800 to turn OFF the instrument. 2. After the power comes back on, start up the UV-1800 normally by following «Installation Precautions» and «Operation Precautions».
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  Warning Labels For safety operation, warning labels are affixed where special attention is required. Should any of these labels peel off or be damaged, obtain replacements from Shimadzu Corporation. (Right side) High Temperature Light source and light source chamber are very hot. When replacing the light…
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  Our company provides a warranty on this product, as stated below. Details 1. Warranty Period: Please consult your Shimadzu representative for information about the extent of the warranty. 2. Warranty Description: If failure occurs for reasons attributable to our company during the warranty period, our company will provide repairs or the replacement of parts without charge (including USB dongles).
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  If the deuterium (D2) lamp should be broken or its life is finished, dispose of the lamp separately from general garbage. When disposing of the deuterium (D2) lamp supplied from Shimadzu Corporation, select a method which will not adversely influence the environment or human body, or ask a special disposal dealer for advice or assistance.
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  The product complies with the requirements of the EMC Directive 2004/108/EC and Low Voltage Directive 2006/95/EC. Product Name: UV-Visible Spectrophotometer Model Name: UV-1800 Manufacturer: SHIMADZU CORPORATION ANALYTICAL & MEASURING INSTRUMENTS DIVISION Address: 1, NISHINOKYO-KUWABARACHO, NAKAGYO-KU, KYOTO, 604-8511, JAPAN Authorized Representative in EU: Shimadzu Europa GmbH Address: Albert-Hahn-Strasse 6-10, 47269 Duisburg, F.R. Germany UV-1800 SERIES…

• Page 13: Table Of Contents

  CONTENTS Chapter 1 General UV-1800 Configuration ……………………1-2 Components ……………………….1-3 1.2.1 UV-1800 Main Body, Front and Top Views …………….1-3 1.2.2 UV-1800 Main Body, Left Side View………………1-4 1.2.3 UV-1800 Main Body, Right Side View ……………… 1-5 1.2.4 Sample Compartment………………….1-6 1.2.5…

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  Calibration Curve …………………… 6-14 6.3.6 Solvent Selection …………………… 6-15 6.3.7 Calibration Curve Curvature ………………..6-16 6.3.8 Spectrophotometer Types………………..6-18 Measurement System……………………6-22 6.4.1 Optical System……………………6-22 6.4.2 Electrical System …………………… 6-24 List of Cells ……………………….6-25 Cleaning the Cell ……………………..6-28 UV-1800 SERIES…

• Page 15: Chapter 1 General

  1 Installation Chapter 1 General CONTENTS UV-1800 Configuration ……………………1-2 Components ……………………….1-3 UV-1800 SERIES…

• Page 16: Uv-1800 Configuration

  Fig. 1.1 Standard Contents Table 1.1 Standard Contents Check Description Part No. Qty. Spectrophotometer 206-25201-91 AC Power Cable 071-60816-12 UV-1800 Instruction Manual-System User’s 206-97040 Guide (This manual) UV-1800 Instruction Manual-Operation 206-97042 Guide UVProve Software (Install CD) 206-21439-91 UVProve Tutorial (Instruction Manual) 206-94459…

• Page 17: Components

  Components 1.2.1 UV-1800 Main Body, Front and Top Views Fig. 1.2 Front and Top Views of UV-1800 Name Description This displays the operation menus and measurement results. Keypad This is the input component for giving operation commands and numeric values to the instrument, etc.

• Page 18: Uv-1800 Main Body, Left Side View

  1.2 Components 1.2.2 UV-1800 Main Body, Left Side View        100-120, 220-240V~  50-60H 140VA  AC IN SIPPER 100-120, 220-240V~ 50-60H 140VA AC IN SIPPER Fig. 1.3 Left Side View of UV-1800 Name…

• Page 19: Uv-1800 Main Body, Right Side View

  1.2 Components 1.2.3 UV-1800 Main Body, Right Side View Fig. 1.4 Right Side View of UV-1800 Name Description USB Connector Plug in the USB memory to this connector. Power Switch Use this switch to turn ON/OFF the instrument. Press the «I» side on the switch to turn ON;…

• Page 20: Sample Compartment

  «4.3 Removing/Installing the Sample Compartment Front Cover» Multi-cell Holder Drive This is the connector for connecting the control cables for multi-cell Connector sample compartment and 8/16-cell micro multi cell holder (MMC-1600) (special accessories). UV-1800 SERIES…

• Page 21: Keypad

  Use these keys to enter numeric values. Use this key to clear a numeric value entry error. When you press this key, the numeric value which has been entered will be cleared and then you may reenter the appropriate value. UV-1800 SERIES…

• Page 22: Light Source Compartment

  The light source switch can be set anywhere in the range from 295 nm to 364 nm in 0.1 nm increments. For details, refer to the Operation Guide, «12.2 Explanation for Setting Instrument Parameters», [4. Light Source Switching Wavelength]. UV-1800 SERIES…

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  Installation Site ……………………..2-2 Connecting Power ……………………..2-4 Checking the Light Source Lamp (D2) ………………..2-7 Operation Precautions ……………………2-8 Turning ON the Power and Initialization ……………….. 2-9 Instrument Baseline Correction ………………….. 2-13 Performance Check after Installation ………………..2-15 UV-1800 SERIES…

• Page 24: Installation Site

  Installation Site 2.1.1 Installation Requirements and Preparation To use the UV-1800 properly and safely, install the instrument in an appropriate location that meets the following requirements. WARNING When using flammable and toxic samples, be sure to install ventilation equipment at installation site.

• Page 25: Installation Space

  • Ensure that there is at least 100 mm of clearance between the left side of the UV-1800 and the wall. The power supply unit and the light source unit cooling fan are located on the left side of the instrument.

• Page 26: Connecting Power

  50-60H 140VA AC IN SIPPER Fig. 2.2 The following table shows the power supply voltage, power consumption, and frequency of the UV-1800. Power Supply Voltage Power Consumption Frequency (Indication on product nameplate) 100 V — 120 V AC, 220 V — 240 V AC…

• Page 27: Connecting To The Power Outlet

  Shimadzu representative. CAUTION Verify that the power switch of the UV-1800 is OFF (i.e., «O» is pressed in) before connecting the electric plug to the outlet. Insert the connector of the power supply cable (accessory) into the power supply connector (Fig.

• Page 28: Grounding

  Ground the instrument to prevent electric shock and provide reliable performance of the UV-1800. The power supply cable supplied with the UV-1800 consists of three wires including a ground wire. When installing the instrument, be sure to insert the cable into the three-wire system outlet.

• Page 29: Checking The Light Source Lamp (D2)

  Ultraviolet light may be generated, a serious health hazard. • If the UV-1800 has been operating before opening the cover, turn OFF the instrument and let it stand until the lamp cools down sufficiently. Touching the lamp when it is still hot will burn you.

• Page 30: Operation Precautions

  • Before turning ON the power switch, check to be sure that nothing is placed in the sample compartment and cell holder. If the power is turned ON when a sample cell is mounted to the UV-1800, the light source energy check and wavelength check may be judged as «NG» since the light beam is obstructed.

• Page 31: Turning On The Power And Initialization

  Turning ON the Power and Initialization 2.5.1 Power ON/OFF Power switch Fig. 2.4 UV-1800 Power Switch Turning ON the Power Press «I» on the power switch (Fig. 2.4) to turn ON the power. The items to be initialized appear on the screen, and start being initialized or checked sequentially.

• Page 32: Initialization Operation

  This icon is displayed when the lamp is ON, and not displayed when it is OFF. A blinking icon indicates that the lamp is OFF with some errors. Fig. 2.5 Initialization screen 2-10 UV-1800 SERIES…

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  If any abnormality is detected, however, «NG» is displayed and the initialization process is terminated. In such a case, note the item for which «NG» is displayed, and turn OFF the instrument. To learn the check points when an error occurred, refer to «5.1 Errors during Initialization». UV-1800 SERIES 2-11…

• Page 34: Switching System Language

  • UV-1800series System Use’s Guide……. P/N 206-97039 • UV-1800series Operation Guide……P/N 206-97041 Switching Procedure (English to Japanese) Turn ON the UV-1800 by pressing the «I» side of the power switch (Fig. 2.4) while holding down the key on the keypad.

• Page 35: Instrument Baseline Correction

  The UV-1800 uses the correction data obtained from 1 when performing 2. Therefore, the steps and shock noises can be removed in a short time. Usually they can be removed only when corrections are performed with the same interval as that used when the measurement data is obtained.

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  The instrument baseline correction is started. Fig. 2.9 During the correction process, a message will be displayed at the bottome of the screen. To terminate the instrument baseline correction, press the key. Fig. 2.10 2-14 UV-1800 SERIES…

• Page 37: Performance Check After Installation

  • Baseline flatness • Wavelength accuracy Use the instrument validation function in order to check the performance of the UV-1800. For details on how to use the function, refer to the Operation Guide, «16. Instrument Validation Function». 2.7.1 Parameter Settings…

• Page 38: Performing Validation

  2.7 Performance Check after Installation 2.7.2 Performing Validation Connect the printer to the UV-1800, and start the validation by the following procedure: Press the [Settings] key in the Parameter Input screen for the instrument validation. Fig. 2.14 In the Settings screen, set [1.Auto Print] and [Print Init.

• Page 39: Chapter 3 Maintenance & Inspection

  3 Maintenance & Inspection Chapter 3 Maintenance & Inspection CONTENTS Inspection and Maintenance………………….3-2 Inspecting the Sample Compartment ………………..3-3 Checking and Resetting the Lamp Usage Time …………….3-4 Replacing the Light Source ………………….. 3-7 Cleaning the Exterior ……………………3-15 UV-1800 SERIES…

• Page 40: Inspection And Maintenance

  Parts». Using parts other than those specified may damage the parts, causing injuries or system malfunction. • DO NOT remove the cover of the UV-1800. Ignoring this may cause injuries or system malfunction. For internal repair of the UV-1800, contact your Shimadzu representative.

• Page 41: Inspecting The Sample Compartment

  Inspecting the Sample Compartment CAUTION DO NOT spill water or organic solvent on the UV-1800. Doing so may result in electrical failure or instrument malfunction. When analyzing liquid sample, check that no solution sample is spilled on the sample compartment before and after measurement.

• Page 42: Checking And Resetting The Lamp Usage Time

  Checking and Resetting the Lamp Usage Time The UV-1800 has a function to record and display the accumulated usage time of the WI (halogen) and D2 (deuterium) lamps used for the light source. Even though the accumulated usage time is saved to the instrument after the power is turned OFF, the data will be cleared if any electrical problem occurs.

• Page 43: Resetting Procedure

  Maintenance screen (Fig. 3.4). Fig. 3.4 The window for selecting the lamp whose usage is to be reset appears. Use the keys to move the cursor to [D2 lamp] (Fig. 3.5), and press the key. Cursor Fig. 3.5 UV-1800 SERIES…

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  The lamp usage time will be reset. Fig. 3.6 You will return to the [Maintenance] screen (Fig. 3.7). Verify that [D2 lamp usage time] is reset to «0 hours». Press the key to return to the Mode menu screen (Fig. 3.3). Fig. 3.7 UV-1800 SERIES…

• Page 45: Replacing The Light Source

  Replacing the Light Source 3.4.1 Light Source Specifications The UV-1800 uses two types of light source lamps: D2 (deuterium) lamp and WI (halogen) lamp. The D2 lamp and WI lamp are used for ultraviolet region (190 nm — light source switching wavelength and visible/near-infrared region (light source switching wavelength — 1100 nm), respectively.

• Page 46: Lamp Replacement Procedure

  • After inserting the WI lamp into the socket, DO NOT forcedly move it to right and left or up and down. The connection part between the pin and glass may crack, which makes it impossible for the lamp to illuminate. UV-1800 SERIES…

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  Fixing screw While sliding the light source compartment cover and lifting it up at an angled (following the arrow direction in Fig. 3.9), remove it from the main body. Fig. 3.9 Removing the light source compartment cover UV-1800 SERIES…
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  D2 lamp before the work. To remove the D2 lamp, refer to » Replacing the D2 lamp» in this section. Remove the WI lamp retainer spring from the top of the WI lamp. Fig. 3.11 3-10 UV-1800 SERIES…
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  Install the light source compartment cover by the reverse procedure of that described in » Removing the light source compartment cover» in this section. Insert the electric plug into the outlet, and switch ON the UV-1800. (Press the «|» side on the switch.) UV-1800 SERIES 3-11…
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  (i.e., «OK» is displayed for all items.) When the Mode menu screen is displayed, follow the procedure in «3.3.2 Resetting Procedure» to reset the WI lamp usage time. Fig. 3.16 3-12 UV-1800 SERIES…
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  Notch (on the socket) Locating lug Fig. 3.18 Insert the electric plug into the outlet, and switch ON the UV-1800. (Press the «|» side on the switch.) Fig. 3.19 UV-1800 SERIES 3-13…
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  (i.e., «OK» is displayed for all items.) When the Mode menu screen is displayed, follow the procedure in «3.3.2 Resetting Procedure» to reset the D2 lamp usage time. Fig. 3.20 3-14 UV-1800 SERIES…

• Page 53: Cleaning The Exterior

  Then, wipe the moisture off with a dry cloth. NOTE DO NOT leave any spilled water on the UV-1800. DO NOT use alcohol or paint thinner solvents for cleaning. Doing so may cause the instrument surface to rust or discolor.

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  3.5 Cleaning the Exterior This page is intentionally left blank. 3-16 UV-1800 SERIES…

• Page 55: Chapter 4 Replacing The Sample Compartment Parts

  4 Replacing the Sample Compartment Parts Chapter 4 Replacing the Sample Compartment Parts CONTENTS Removing/Installing the Cell Holder………………..4-2 Removing/Installing the Sample Compartment Unit (Standard) …………4-4 Removing/Installing the Sample Compartment Front Cover …………. 4-7 UV-1800 SERIES…

• Page 56: Removing/Installing The Cell Holder

  Standard cell holder Knurled screw Fig. 4.1 Sample compartment (standard) 4.1.1 Removing the Cell Holder Open the sample compartment cover. Loosen the knurled screw fixing the cell holder. Remove the cell holder. Knurled screw Cell holder Fig. 4.2 UV-1800 SERIES…

• Page 57: Installing The Cell Holder

  NOTE Install the cell holder so that the beam passes through it as directed by the arrow mark. Cell holder Fix the cell holder with the knurled screw. Locating pin Locating hole Fig. 4.3 UV-1800 SERIES…

• Page 58: Removing/Installing The Sample Compartment Unit (Standard)

  Fig. 4.4 Main body front view 4.2.1 Removing the Sample Compartment Unit Loosen two of the sample compartment unit fixing Fixing pin (on the UV-1800) screws located at the bottom of the sample Notch (on the sample compartment.

• Page 59: Installing The Sample Compartment Unit

  Open the sample compartment cover and install the sample compartment unit. At an angle from above, insert the notch on Locating pin Notch (on the sample (on the UV-1800) the sample compartment unit to the locating compartment unit) pin at the far side of the sample compartment.

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  4.2 Removing/Installing the Sample Compartment Unit (Standard) Make sure that the sample compartment unit front cover and the UV-1800 main body fit together snugly. If they don’t, press the sample compartment unit down to the sample compartment bottom. Fig. 4.9…

• Page 61: Removing/Installing The Sample Compartment

  Fig. 4.11 Front plate for syringe sipper (switching unit) 4.3.1 Removing the Sample Compartment Front Cover and Installing the Front Plate Remove the sample compartment unit from the UV-1800 by the procedure described in «4.2.1 Removing the Sample Compartment Unit».

• Page 62: Installing The Sample Compartment

  4.3 Removing/Installing the Sample Compartment Front Cover Install the sample compartment unit (without cover) to the UV-1800 main body by the procedure described in «4.2.2 Installing the Sample Compartment Unit». Install the designated front plate (special accessory) to the sample compartment unit.

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  Snap the sample compartment front cover into the sample compartment unit. Push the cover in the arrow direction in Fig. 4.17 until it snaps. Install the sample compartment unit to the UV-1800 main body by the procedure described in «4.2.2 Installing the Sample Compartment Unit».
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  4.3 Removing/Installing the Sample Compartment Front Cover This page is intentionally left blank. 4-10 UV-1800 SERIES…
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  5 Troubleshooting Chapter 5 Troubleshooting CONTENTS Errors during Initialization ……………………5-2 Problems: Symptoms and Solutions………………..5-4 UV-1800 SERIES…

• Page 66: Errors During Initialization

  Errors during Initialization After the power is turned ON, the UV-1800 initializes and checks the following items listed in the table below. Fig. 5.1 Initialization screen Table 5.1 Initialization items Item No. Initialization items Description LSI Initialize Initializes each I/O device.

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  5.1 Errors during Initialization When [NG] is displayed during initialization, perform the appropriate remedial actions by referring to the table below. If the problem is not resolved by these actions, contact your Shimadzu representative. Reference Item No. Check Point Remedial Action…

• Page 68: Problems: Symptoms And Solutions

  Problems: Symptoms and Solutions Check whether the problem exhibits the following symptoms before requesting a repair. Contact your Shimadzu representative if the error cannot be resolved through the remedial action described below, or if the symptom is not covered in the table.

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  The lamp is currently OFF with some errors. The fan is not working or the thermo —— censor on the circuit board has detected overheating. Turn OFF the UV-1800 power switch and contact your Shimadzu representative. Did you mistakenly Return to the blank condition press the…
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  «Cannot load this file.» Has the file been The files once saved in the UVProbe saved in the cannot be loaded on the UV-1800. —— UVProbe? Has the file been The selected file may be corrupted. properly saved? In that case, the file cannot be ——…

• Page 71: Chapter 6 Reference

  6 Reference Chapter 6 Reference CONTENTS Specifications ………………………. 6-2 Maintenance Parts……………………..6-8 Spectrophotometer Basics …………………… 6-9 Measurement System……………………6-22 List of Cells ……………………….6-25 Cleaning the Cell ……………………..6-28 UV-1800 SERIES…

• Page 72: Specifications

  15 °C ~ 35 °C Operating Humidity 30 % ~ 80 % (No condensation, 70 % or less at 30 °C or higher) Power Supply AC 100 V/120 V/220 V/230 V/240 V, 50/60 Hz Power Consumption 140 VA UV-1800 SERIES…

• Page 73: Software Specifications

  — 0.001 to 0.001) %T, E; -400.0 to 400.0 (Min. range; — 0.1 to 0.1) (5) Scan repetitions: 1 to 99 (6) Recording method: Overlay/ Sequential selectable (7) Reduce/Expand [zoom], Display data with cursor (8) Save/Load data function UV-1800 SERIES…

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  (3) Measurement using Abs difference between two wavelengths is possible. Rate-measurement (1) Measure Abs time change and calculate the change in absorbance quautity. (2) Measurement time: 1 to 9999 sec/min (3) Determine whether the measured reaction result is progressing in linear fashion. UV-1800 SERIES…
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  DNA concentration =K1×A1-K2×A2 Protein concentration = K3×A2-K4×A1 (2) Factor/measurement wavelength can be set arbitrarily. (3) Background correction can be applied. Protein quantitation (1) Quantitation method: Lowry method, BCA method, Biuret method, CBB method (Bradford method), UV absorption method UV-1800 SERIES…
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  • Peak/Valley detection (both possible up to 20 pcs.) • The four rules of arithmetic • Derivative processing • Smoothing processing • Area calculation • Point-pick processing • Print processing (printing waveform data in text format) UV-1800 SERIES…
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  : Max. 8 files (4) Accumulation time set function (for fixed wavelength measurement) (5) External control function UV-1800 can be controlled from PC. This function is applied for the control with bundled UVProve software. * USB cable (that supports USB 1.1) separately required…

• Page 78: Maintenance Parts

  Fuse, 218 004 072-02004-22 For 100-120 V, 220-240 V Battery, CR2032H 38 074-73306-08 O-ring 036-15501-21 Used for fixing the quartz plate. Alternate Sample Compartment 206-60184-07 Standard cell holder 206-82009-91 Sample compartment front cover 206-25269 ASSY Sample compartment cover 206-25269 UV-1800 SERIES…

• Page 79: Spectrophotometer Basics

  Ultraviolet rays (vacuum ultraviolet) 50 nm X-rays 5 pm γ-ray (0.05 Å) (Note) 1 mm = 10 m, 1 µm = 10 m, 1 nm = 10 m, 1 pm = 10 Fig. 6.1 Types of electromagnetic waves UV-1800 SERIES…

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  580 — 595 Yellow Blue 480 — 490 Greenish blue Orange 595 — 610 Orange Greenish blue 490 — 500 Bluish green 610 — 680 Bluish green 500 — 560 Green Purplish red 680 — 700 Purplish red Green 6-10 UV-1800 SERIES…

• Page 81: Ultraviolet/Visible Spectrum

  In addition, the molecule may emit the absorbed light energy as light when returning to the ground state. Fluorescent light and phosphorescence exhibit the behavior and analysis utilizing these phenomena is called fluorometry. Excited state 1 Excited state 2 Light Ground state Fig. 6.4 Molecule energy UV-1800 SERIES 6-11…

• Page 82: Bouguer-Beer’s Law

  ε. This molar absorptivity becomes a value unique to the substance under specific conditions. To fulfil Bouguer-Beer’s law, it is necessary to satisfy conditions such as being free from stray light, emission, scattering, and reflection. 6-12 UV-1800 SERIES…

• Page 83: Qualitative Analysis And Quantitative Analysis

  Analysis to perform quantitative analysis by comparing the color darkness of a substance is called colorimetric analysis. When the substance is transparent, if absorbance exists in the invisible ultraviolet/near infrared area, it is measured. The latter is broadly included in colorimetric analysis. UV-1800 SERIES 6-13…

• Page 84: Calibration Curve

  This method is applied only when the related curve is straight as far as to the low concentration range. Generally, extra-large absorbance wavelength is used as measurement wavelength for quantitative analysis. 6-14 UV-1800 SERIES…

• Page 85: Solvent Selection

  Dichloromethane Chloroform Metylacetate Metyl formic acid Carbon tetrachloride Dimethyl sulfoxide Benzene Xylene Toluene Ethylene tetrachloride Acetone Bromoform Nitro methane Carbon disulfide 450 (nm) Fig. 6.7 Wavelength range for solvent to be used (using a 10 mm cell) UV-1800 SERIES 6-15…

• Page 86: Calibration Curve Curvature

  0.0211 0.1791 0.002 0.0000 0.0000 0.0001 0.0003 0.0005 0.0008 0.0027 0.0085 0.0791 0.001 0.0000 0.0000 0.0000 0.0001 0.0002 0.0004 0.0013 0.0043 0.0413 0.0001 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0001 0.0004 0.0043 (Test and Engineering Vol.9 No.9,702,1981) 6-16 UV-1800 SERIES…

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  If the bandwidth is made large, the peak height becomes low in appearance and this may cause the calibration curve to be bent. Spectrum band width Real spectrum half-width Fig. 6.9 Band width and peak height UV-1800 SERIES 6-17…

• Page 88: Spectrophotometer Types

  I and the reference sign Io simultaneously. Reference side Cell Light source Monochromator Detector Amplifier Display Sample side (Rotating mirror, semi- Sample compartment transparent mirror) Fig. 6.11 Double beam configuration 6-18 UV-1800 SERIES…

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  Deuterium lamp Window plate S1-2: Slit L.M.: Light-source switching mirror M1-5: Mirror Grating Filter B.S.: Beam splitter Sam: Sample side cell Ref: Reference side cell Lens P.D.: Photodiode Fig. 6.13 Double beam — double detector configuration (UV-1800) UV-1800 SERIES 6-19…
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  Halogen lamp Deuterium lamp Window plate S1-3: Slit M1-12: Mirror G1-2: Grating Filter Chopper mirror Photomultiplier Fig. 6.14 Double monochromator configuration (UV-2500 series) 6-20 UV-1800 SERIES…
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  Sample side cell (First monochromator) W1 ~ W3: Window plate (ø 30 mm) G3, G4: Diffraction grating W4 ~ W5: Window plate (ø 40 mm) (Second monochromator) C.H.: Chopper mirror Fig. 6.15 Double monochromator configuration (UV-3600 series) UV-1800 SERIES 6-21…

• Page 92: Measurement System

  Measurement System 6.4.1 Optical System A schematic of the optical system for the UV-1800 is shown in Fig. 6.16. L.M.: Light-source switching mirror Deuterium lamp M1~M5: Mirrors Halogen lamp B.S.: Beam splitter Filter Lens Grating Sam: Sample cell Entrance slit…

• Page 93
  The monochromator slit aperture is fixed at 1 nm. The Shimadzu blazed holographic grading is used for the diffraction grading. The light coming out of the monochromator passes through a stray light cutting filter F and strikes the mirror M3 and is then split by the beam splitter B.S.

• Page 94: Electrical System

  6.4 Measurement System 6.4.2 Electrical System A schematic of the electrical system for the UV-1800 is shown in Fig. 6.18. The center of control is the microcomputer (CPU), which performs all controls of light sources, switching of light sources, filter switching, wavelength scanning pulse motor, LCD monitor display, keyboard and printer, USB interface etc.

• Page 95: List Of Cells

  Micro multi-cell (8 cells) optical length 10 mm and holder req’d 208-92089 required sample volume of 100 µl more (206-23680-91 206-23690-91) Micro multi-cell Micro multi-cell (16 cells) optical length 10 mm holder req’d 208-92088 and required sample volume of 100 µl more (206-23680-91 206-23690-91) UV-1800 SERIES 6-25…

• Page 96
  Standard required sample volume: 1.0 ml or more Square flow cell (semi-micro) for syringe sipper, light path length: 10 mm 208-92005 390 µl 3.5 × 11 Unnecessary With tube Standard required sample volume: 5.0 ml or more 6-26 UV-1800 SERIES…
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  6.5 List of Cells 9 mm pitch 4.5 mm pitch unit : mm Fig. 6.19 Optional Cell Shapes UV-1800 SERIES 6-27…

• Page 98: Cleaning The Cell

  «PVC tube for peristaltic pump» since the tube corrodes and hardens from the solvent. Be sure to fill the flow cell with distilled water after washing. If the flow cell is left empty, the cell may get stained and it becomes hard to clean afterwards. 6-28 UV-1800 SERIES…

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  Record of Revision Date Revision Changed Page Description 2/2008 Front, 2-15, 6-2, 6-8, and more A: Record of Revision Note) A …Added Page No. D …Deleted Page No.

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