Bio rad imark microplate reader инструкция

1 iMark Microplate Absorbance Reader Instruction Manual i Warranty Statement This warranty may vary outside of the continental United States. Contact your local Bio-Rad office for the exact terms of your warranty. Bio-Rad Laboratories warrants to the customer that the iMark Microplate Absorbance Reader will be free from defects in material and workmanship, and will meet all performance specifications for a period of one year from the date of shipment. This warranty covers all parts and labor. In the event that the instrument must be returned to the factory for repair under warranty, the instrument must be packed for return in original packaging. Please contact your local Bio-Rad representative for the necessary return documents. The required documentation includes a decontamination certificate.

2 Bio-Rad shall not be liable for any incidental, special, or consequential loss, damage, or expense directly or indirectly arising from the use of the iMark Microplate Absorbance Reader . Bio-Rad makes no warranty whatsoever in regard to products or parts furnished by third parties, such being subject to the warranty of their respective manufacturers. Service under this warranty shall be requested by contacting your nearest Bio-Rad office. The following items are considered customer-installable consumables: thermal printer paper and light bulbs. These parts are not covered by this warranty. All customer-installed parts are warranted only to be free from defects in workmanship. This warranty does not extend to any instrument or parts thereof that have been subject to misuse, neglect, or accident, or that have been modified by anyone other than Bio-Rad or that have been used in violation of Bio-Rad instructions .

3 The foregoing obligations are in lieu of all other obligations and liabilities including negligence and all warranties of merchantability, fitness for a particular purpose, or otherwise expressed or implied in fact or by law, and state Bio-Rad s entire and exclusive liability and buyer s exclusive remedy for any claims or damages in connection with the furnishing of goods or parts, their design, suitability for use, installation or operation. Bio-Rad will in no event be liable for any special, incidental or consequential damages whatsoever, and Bio-Rad s liability under no circumstances will exceed the contract price for the goods for which liability is claimed. ii Regulatory Notices Electro Magnetic Compatibility: This instrument is designed to meet the Class-A emissions and immunity levels of the EN 61326 product family standard for the CE mark.

4 This rating requires that it only be used in a laboratory environment. NOTE: These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the Instruction manual, may cause harmful interference to radio communications. Operation of equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his/her own expense. Safety This instrument is designed to meet EN 61010-1 requirements for the CE mark, which are internationally accepted safety standards for electrical equipment for measurement, control and laboratory use. EN 61010-1 applies to equipment which is used under the following conditions: Indoor use Altitude up to 2,000 m MAINS supply voltage fluctuations up to 10% This instrument has been tested, and its compliance has been confirmed under the following environmental conditions: Overvoltage category II Pollution degree 2 If the instrument is used in a manner not specified in this manual, the protection provided by the instrument may be impaired.

5 This instrument should not be modified or altered in any way. Alteration of this instrument will result in the following: Void the manufacture s warranty. Void regulatory compliance. Create a potential safety hazard. Protective Ground This instrument has a detachable, three-wire power cord for connection to both the power source and protective ground. The protective ground contact is connected to the accessible metal parts of the instrument. To prevent electrical shock, always use a power source outlet that has a properly grounded protective-ground contact. iii Table of Contents USB Built-in External Keypad Instrument Unpacking the Initial Instrument Instrument Software Quick Guide to Reading a Instrument Security Memory Limits ..9 Report End-point Protocol 1.

6 Raw data 2. Absorbance 3. Limit 4. Matrix 5. Cutoff 6. Curve Fit 7. Concentration 8. Difference 9. TeSeE Screening Kinetic Protocol 1. Absorbance 2. Kinetic Plots 3. Linear Regression 4. GALT iv Screen by Screen Initial Configuring the Printing Reports and Protocol Memory Recall Editing the Kinetic Checkmark Value Setting (Administrator only)..24 Editing End Point Cutoff Setting Standard Setting Mode Setting Plate Map Setting Instrument Installing Interference Changing the Troubleshooting and Error Instrument iMark Microplate Absorbance Reader Instruction Manual 1 Introduction Description The iMark Microplate Absorbance Reader (catalog #168-1130) is an eight-channel, vertical pathlength photometer that measures the Absorbance of the contents in the wells of a 96-well microtitration plates.

7 It can perform single or dual wavelength measurements and can report Absorbance values to three decimal places. The iMark Reader can be programmed by entering commands through the membrane keypad to define plate reading and data analysis parameters as testing protocols and select report types. Hard copy reports can be produced by an optional on-board thermal printer or external printer. The iMark Reader can also be controlled by the Windows-based Microplate Manager software (catalog # 168-9520) program, through its built-in USB interface device. Microplate Manager software provides a complete, user-friendly solution for protocol programming as well as data analysis, for all Microplate Absorbance readers from Bio-Rad Laboratories. USB Interface The iMark Microplate Reader has a built-in USB interface. This allows external computers to control the instrument.

8 When an external computer is in control of the Microplate Reader , the printer and the membrane keys, except for the Start/Stop and Open/Close keys, are automatically deactivated, and the LCD display appears as below. The Reader will remain in remote control mode until it is released or until the Start/Stop key is pressed on the Reader . Built-in Printer The iMark Microplate Reader has a built-in printer. It can generate all types of reports, including graphs and kinetic plots. Remote mode iMark Microplate Absorbance Reader Instruction Manual 2 Catalog # Description 168-1130 iMark Microplate Absorbance Reader with built-in printer 168-1135 iMark Microplate Absorbance Reader with built-in printer, including Microplate Manager 6 software 168-6940 Checkmark Absorbance Reader Performance Validation Kit Accessories Catalog # Description 168-1011 405 nm filter 168-1013 415 nm filter 168-1020 450 nm filter 168-1028 490 nm filter 168-1038 540 nm filter 168-1040 550 nm filter 168-1044 570 nm filter 168-1049 595 nm filter 168-1054 620 nm filter 168-1056 630 nm filter 168-1061 655 nm filter 168-1080 750 nm filter 168-1006 Replacement lamp for iMark Microplate Absorbance Reader 168-2007 Replacement printer paper, package contains 3 rolls Custom filters between 400 and 750 nm may be ordered.

9 Corresponding catalog numbers can be found on , or you can order by specifying the wavelength and the model number of the Reader . Checkmark Absorbance Reader Performance Validation Kit (catalog item 168-6940) consists of a plate with Absorbance standards and software to calculate the accuracy and precision of the iMark Microplate Absorbance Reader . iMark Microplate Absorbance Reader Instruction Manual 3 External Features Release latch for rear cover Rear cover Printer slot Power button Liquid crystal display (LCD) Keypad Reading chamber door Power cord receptacle Serial number label Cooling vents and fan USB interface iMark Microplate Absorbance Reader Instruction Manual 4 Keypad Guide Main Returns to MAIN SCREEN. Start/Stop Initiates plate reading using current active protocol.

10 Stops plate reading and printing. Paper Feed Advances paper strip in internal printer. Print Prints out the plate data and protocol information. Edit Enters the Edit Menu, and sets up the instrument. Memory Recall Reads out protocol and plate data. Open/Close Opens or closes the reading chamber door. Up Arrow Moves the cursor upward. Selects the alphabet or the symbol. Left Arrow Returns to the previous screen. Moves the cursor to the left. Down Arrow Moves the cursor downward. Selects the alphabet or the symbol. Right Arrow Moves the cursor to the right. Changes or selects the value and type. Enter Completes or seals a field entry. Dot/Function Inputs the dot. Changes the input mode. Ten Keys Inputs numbers or well type in plate mapping. 0 / EMP : Empty 5 / QC : QC control 1 / SMP : Sample 6 / CAL : Calibrator 2 / BLK : Blank 7 / CP : Positive Control 3 / STD : Standard 8 / CN : Negative Control 4 / CO : Cutoff control 9 / CW : Weak Positive Control Open/Close Edit Print Paper Feed Main START / STOP Memory Recall7 CP 8CN9CW4 CO 5QC6 CAL2 BLK3 STD0 EMP 0 FUNCSMP 1 Change ENTER Back Z = A > A = Z > + iMark Microplate Absorbance Reader Instruction Manual 5 Instrument Setup Unpacking the Instrument The shipping carton contains the following items.

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• Life Science Group

  Model 680 and 680 XR Microplate Reader

  Service Manual

  Part Number 100-2414 Revision E

• 100-2414E 02/06 i

  PROPRIETARY NOTICE

  Information contained in this manual is copyrighted by Bio-Rad
  Laboratories, Inc. None of the contents may bereproduced in full or
  in part by any person without the prior written approval of Bio-Rad
  Laboratories, Inc. Thismanual is intended to provide information to
  assist the qualified field or instrument service technician to
  repair,maintain, and order replace parts for the instrument
  supplied. Every reasonable effort has been made to keepthe
  information in this manual current and accurate as of the date of
  publication or revision. However, no guar-antee is given or implied
  that the manual is error-free or accurate about any
  specification.

  Copyright 2002-2006 by Bio-Rad Laboratories, Incorporated. All
  rights reserved.

  Bio-Rad Laboratories Inc, Life Science Group

  2000 Alfred Nobel Drive

  Hercules, California 94547

  Telephone: 1-800-424-6723

• ii 100-2414E 02/06

  About this manual This document is part of Life Science Groups
  commitment to provideyou, the service professional, with adequate
  information to effectivelysupport Bio-Rad instruments. Additional
  copies of this service documentmay be ordered using part number
  100-2414.

  We welcome and appreciate any suggestions you may have to
  improveour service documentation. Please address your comments or
  sugges-tions to:

  Technical Editor, Global Service Support Dept.

  Bio-Rad Laboratories, Life Science Group

  2000 Alfred Nobel Drive

  Hercules, CA 94547

  FAX Number: 510 741-5802

• 100-2414E 02/06 iii

  Revision Control List

  Document: Model 680 and 680 XR Microplate Reader Service
  ManualPart Number: 100-2414Date of Latest Revision: February
  2006

  Each page of this manual has the catalog number, revision level
  and revision date for that page. Subsequentrevisions to a page will
  be indicated by changes to the revision level and revision date for
  that page. A sum-mary of changes for all revisions is listed in the
  following table.

  RevisionPage

  NumberDescription

  ChangedBy

  Date

  A — Initial Release A. Gandy Feb. 2002

  B 4-11 to 4-12 Updated Checkmark Plate procedure A. Gandy June
  2002

  5-1 to 5-4 Added optics cleaning procedure

  6-1 to 6-25 Added part numbers to sections; Updated Figure
  6-9

  6-16 Added check of plate position

  7-1 to 7-2 Added Checkmark Plate; Updated Figure 7-3

  7-4 Added Section AdjustingPlate Carrier Belt Tension

  7-6 Added Section Alignment of Plate Carrier

  8-5 Added Table 8-4 Checkmark Plate Failures

  C Sec 1 thru 10 Manual Updated to Add Model 680XR A. Gandy Mar
  2004

  5-1 Added Note

  6-3 Added 6.3.3 Model 680 XR Lamp

  6-12 Added Table 6-1

  6-15 to 6-16 Added 6.3.11 Model 680 XR Lamp Control PCB

  6-22 Added 6.3.17 Model 680 XR Light Balancing Filter

  7-8 Added Table 2 Firmware History

  8-1 Updated Table 8-1

  9-3 to 9-11 Updated parts information

  App D Added Appendix D

  D 6-12 Added Backlit LCD to Table 6-1 A. Gandy Sept 2005

  7-6 Updated to reference Section 7.7

  7-9 Updated Firmware Table 7-2 and added 7.6.3

  7-10 to 7-11 Added Section 7.7 QC Software

  9-5 to 9-11 Updated parts

  E 7-8 to 7-9 Updated Firmware version 1.71; Table 7-2 update A.
  Gandy Feb 2006

  7-11 to 7-15 Updated 7.6.4 Firmware Downloading

  7-17 Added Section 7-8 External Printer Setup

  8-1 Updated troubleshooting printer

  9-11 Added Shield Cover

• Table of Contents

  Section 1 Introduction 1.1 General Inforation . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . 1-1

  1.2 Service Philosophy . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  1-1

  1.3 Safety and Precautions . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  1-1

  1.4 Conventions . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . 1-1

  Section 2 System Overview 2.1 Introduction . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . 2-1

  2.2 Product Description . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-1

  2.3 Product Configurations . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-2

  2.4 Regulatory Notices . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-2

  2.5 Product Overview . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-3

  2.5.1 Calculations . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

  2.5.2 Limits . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-3

  2.5.3 Memory Backup . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

  2.5.4 Reports . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-4

  2.5.5 RS-232 Interface . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

  2.6 Instrument Specifications . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-5

  2.6.1 Features . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

  2.6.2 Optical . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-5

  2.6.3 Performance . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

  2.6.4 Thermal . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-6

  2.6.5 Electrical . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-6

  2.6.6 Physical . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  2-6

  Section 3 Installation 3.1 Introduction . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . 3-1

  3.2 Visual check . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . 3-1

  3.3 Installation Procedure . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  3-2

  3.4 Install Paper Roll (Optional Internal Printer) . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

  3.5 Computer Interface (Optional). . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

  Section 4 System Checkout 4.1 Introduction . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . 4-1

  4.2 Tools and Materials . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  4-1

  4.3 Summary of Tests and Specifications . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

  4.4 System Checkout Procedure . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

  4.4.1 General. . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  4-3

  4.4.2 Setup . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  4-3

  4.4.3 Preparing Yellow Dye Test Plate . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . 4-3

• Table of Contents

  4.4.4 Startup . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  4-3

  4.4.5 Touch Pad/Mixing Function . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

  4.4.6 Verify Printer . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

  4.4.7 Start Microplate Manager. . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

  4.4.8 Air Read . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  4-5

  4.4.9 Accuracy . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7

  4.4.10 Channel-to-Channel Variation . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 4-8

  4.4.11 Stability and Drift . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9

  4.4.12 Verify Incubator Temperature (Optional) . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . 4-10

  4.5 Checkmark Validation Program (Optional Method) . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . 4-11

  4.5.1 General . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  4-11

  4.5.2 Procedure. . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  4-11

  Section 5 Preventative Maintenance5.1 Introduction . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . 5-1

  5.2 Tools and materials

  5.3 Preventative Maintenance. . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  5-1

  5.3.1 Firmware . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

  5.3.2 Inspection of Instrument . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

  5.3.3 Perform System Checkout . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

  Section 6 Removal and Replacement 6.1 Introduction . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . 6-1

  6.2 Tools and Materials . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  6-1

  6.3 Removal and Replacement Procedures . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . 6-1

  6.3.1 Interference Filters . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

  6.3.2 Model 680 Lamp . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

  6.3.3 Model 680 XR Lamp . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3

  6.3.4 Top Cover . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4

  6.3.5 Printer Assembly . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5

  6.3.6 Keypad Membrane. . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 6-7

  6.3.7 Inlet Assembly . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-8

  6.3.8 Plate Carrier Assembly and Analog Board (#282) . . . . . .
  . . . . . . . . . . . . . . . . . . 6-9

  6.3.9 CPU Board (#281) . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-12

  6.3.10 Incubator Control Board . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-13

  6.3.11 Model 680 XR Lamp Control PCB . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . 6-15

  6.3.12 Power Supply, 12 Volt . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-17

  6.3.13 Power Supply, 24 Volt . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-18

  6.3.14 Photo Sensor (Analog Board) . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . 6-19

• Table of Contents

  6.3.15 Photo Sensor (Filter Wheel) . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 6-20

  6.3.16 Model 680 Light Balancing Filter . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 6-21

  6.3.17 Model 680 XR Light Balancing Filter . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . 6-22

  6.3.18 Plate Carrier Motor . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-23

  6.3.19 Optical Fiber Assembly . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-25

  6.3.20 Filter Wheel/Filter Wheel Motor . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 6-27

  6.3.21 Fan . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  6-28

  6.3.22 Lithium Battery . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  6-29

  Section 7 Adjustment and Calibration 7.1 Introduction . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . 7-1

  7.2 Tools and Materials . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  7-1

  7.3 Electronics Adjustment . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  7-1

  7.3.1 Setting of Jumper on the CPU Board . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . 7-1

  7.3.2 Setting of Jumpers on the Printer Control Board . . . . .
  . . . . . . . . . . . . . . . . . . . . 7-2

  7.3.3 Incubator Electronics Adjustment . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . 7-3

  7.4 Adjusting Plate Carrier Belt Tension . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4

  7.5 Plate Carrier Alignment . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  7-6

  7.5.1 Checking of Plate Alignment . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 7-6

  7.5.2 Adjusting Plate Alignment — Y Direction . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . 7-7

  7.5.3 Adjusting Plate Alignment — X Direction . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . 7-7

  7.6 Firmware . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . 7-8

  7.6.1 General . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  7-8

  7.6.2 Firmware History . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-9

  7.6.3 Integration of English and Chinese Firmware Version . . .
  . . . . . . . . . . . . . . . . . . 7-10

  7.6.4 Downloading Firmware. . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 7-11

  7.7 QC Software . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . 7-16

  7.7.1 Entering QC Software . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16

  7.7.2 Monitor Mode . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16

  7.7.3 Plate Positioning. . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-17

  7.8 External Printer Setup . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  7-17

  Section 8 Troubleshooting 8.1 About this Section . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . 8-1

  8.2 Troubleshooting . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  8-1

  Table 8-1 General Problems . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1

  Table 8-2 Error Codes. . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-2

  Table 8-3 Firmware Downloading . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 8-4

• Table of Contents

  Section 9 Parts Information9.1 About this Section . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . 9-1

  9.2 Parts Orders . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . 9-1

  9.2.1 US Domestic Operations . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

  9.2.2 International Subsidiaries . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

  9.3 Returning Defective Parts . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  9-1

  9.3.1 US Domestic Operations . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

  9.3.2 International Subsidiaries . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2

  9.4 Service Parts . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . 9-3

  Table 9-1 Replaceable Parts for the Model 680 Microplate Reader
  . . . . . . . . . . . . . . . . 9-3

  Table 9-2 Consumables . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-11

  9.5 Filters . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . 9-12

  Table 9-3 Filters . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  9-12

  9.6 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . 9-13

  Table 9-4 Special Service Tools . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-13

  9.7 Manuals . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . 9-14

  Table 9-5 Manuals . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  9-14

  Section 10 Drawings 10.1 About this Section . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . 10-1

  10.2 Drawings . . . . . . . . . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
  . 10-1

  Block Diagram Wiring Diagram

  AppendixAppendix A Model 680 System Checkout Report . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . A-1

  Appendix B Model 680 XR System Checkout Report . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . B-1

  Appendix C Checkmark Check Plate . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . C-1

  Appendix D Error Flow Diagrams . . . . . . . . . . . . . . . . .
  . . . . . . . . . . . . . . . . . . . . . . . . . . D-1

• Introduction

  100-2414C 03/04 1-1

  Section 1Introduction

  1.1 General Information

  This manual is the reference to be used for troubleshooting,
  repair, and maintenance of the Model 680 andModel 680 XR Microplate
  Readers. It is intended to be used by trained technicians and
  engineers familiar withsafety precautions relative to working with
  instrumentation. The procedures in this manual assume that
  thereader is familiar with the operation of the Model 680 and 680
  XR Microplate Readers. Complete operatinginstructions are contained
  in the Model 680 and 680 XR Microplate Reader instruction manual.
  Informationpresented in the instruction manual is not repeated in
  this Service Manual unless there is a compelling reasonto do
  so.

  1.2 Service Philosophy

  This manual is designed to support mail in products. Regional
  service centers are the first level of service,where faulty
  assemblies can be most effectively repaired at a central repair
  facility. The second level of serviceis performed at our factory
  instrument service facility in Richmond, California, where
  additional service proce-dures requiring resources not available at
  regional service centers are performed.

  1.3 Safety and Precautions

  The Model 680 and 680 XR Microplate Reader contains the
  following Hazards:

  Hazardous voltages

  Thermally hot components

  The following should be observed when servicing this unit:

  When power is required, the unit should be plugged into an
  outlet with a properly grounded receptacle.The use of 2-prong plug
  adapters is not recommended and will void the safety certification
  of thisproduct.

  The use of extension cords may compromise the safety of the
  operator and is not recommended.

  Ensure that fuses installed in the unit are of the correct
  rating.

  Ensure that none of the ventilation openings in the unit are
  blocked. Excessive heat buildup in the unitmay cause failures.

  Bio-Rad is not responsible for any injury or damage caused by
  the use of this instrument for purposes otherthan for which it is
  intended or by modifications of the instrument not performed by
  Bio-Rad or an authorizedagent.

  1.4 Conventions

  The following conventions are used throughout this manual.:

  Left and right sides of the unit are as viewed from the front,
  operators position, unless otherwise stated.

  Dimensions are in millimeters, unless otherwise stated.

• System Overview

  100-2414C 03/04 2-1

  Section 2System Overview

  2.1 Introduction

  The Model 680 and Model 680 XR Microplate Readers are an
  eight-channel, vertical pathlength photometerthat measures
  absorbance of the contents of 96 well mictotitration plates. They
  can perform single or dualwavelength measurements, and can report
  absorbance values to three decimal places.

  The Model 680 and 680 XR Microplate Readers can be programed by
  entering commands through the keypadto define plate reading
  conditions and data analysis conditions as a protocol data, and
  select report types. Aprinted report is produced by an optional
  on-board thermal printer or external printer. An optional incubator
  isalso available.

  The Model 680 and 680 XR Microplate Readers feature a built-in
  RS-232C serial interface device for conve-nient computer
  interfacing. The Microplate Manager software for personal computers
  offers a complete analy-sis program. Microplate Manager supports
  Windows 95, Windows NT and Windows 2000.

  Microplate Manager Version 5.2.1 added support for the Model
  680XR reader.

  2.2 Product Description

  Figure 1: Model 680/680 XR Microplate Reader

  The following items are included with the Model 680/680 XR
  Microplate Reader system:

  Microplate Reader with 415 nm, 450 nm, 490 nm and 655 nm
  interference filters installed

  Power Cord

  Spare Fuse

  Dust Cover

  Instruction Manual

  Warranty Card

• System Overview

  2-2 100-2414C 03/04

  2.3 Product Configurations

  The Model 680 Microplate Reader is available in four
  configurations.

  2.4 Regulatory Notices

  This instrument is designed to meet Class A emissions and
  immunity levels of EN61326 product family stan-dard for CE mark.
  This rating requires that it only be used in a laboratory
  environment.

  This instrument is designed and certified to meet EN61010
  requirements which are internationally acceptedelectrical safety
  standards. Certified products are safe to use when operated in
  accordance with the InstructionManual. This instrument should not
  be modified or altered in any way. Alteration of this instrument
  will result invoiding of the manufacturers warranty, void
  regulatory certification and create a potential safety hazard.

  This equipment has been tested and found to comply with the
  limits for Class A digital device, pursuant to Part15 of FCC rules.
  These limits are designed to provide a reasonable protection
  against harmful interferencewhen the equipment is operated in a
  commercial environment. This equipment generates, and can
  radiateradio frequency energy and if not installed and used in
  accordance with the Instruction Manual, may causeharmful
  interference to radio communications. Operation of equipment in a
  residential area is likely to causeharmful interference in which
  case the user will be required to correct the interference at their
  expense.

  Table 1: Product Configurations

  Catalog Number Description

  168-1000 Model 680 Microplate Reader No Temperature Control and
  No Internal Printer

  168-1001 Model 680 Microplate Reader With Temperature Control
  and No Internal Printer

  168-1002 Model 680 Microplate Reader No Temperature Control and
  With Internal Printer

  168-1003 Model 680 Microplate Reader With Temperature Control
  and With Internal Printer

  168-1100 Model 680 XR Microplate Reader No Temperature Control
  and No Internal Printer

  168-1101 Model 680 XR Microplate Reader With Temperature Control
  and With Internal Printer

• System Overview

  100-2414C 03/04 2-3

  2.5 Product Overview

  2.5.1 Calculations

  The Model 680 and 680 XR Microplate Readers uses Beers Law to
  calculate the absorbance value of each well. Beers Law states that
  absorbance is equal to the Log10 of the ratio on the baseline
  measurement intensity (Io) to the Sample measurement intensity
  (I).

  Beers Law: Absorbance = Log10 (Io/I)

  Before measuring the plate, the reader takes a reading for all
  eight photodiode channels. These values are recorded as the
  baseline measurement (Io) values for each channel, respectively.
  The reader then records the sample measurement (I) value for each
  well, and calculates the absorbance using these values.
  Chan-nel-to-channel error is significantly reduced because the Io
  value for a given channel is used only in deter-mining the
  absorbance of the wells of that channel.

  2.5.2 Limits

  The Model 680 and 680 XR Microplate Readers display absorbance
  readings with absolute values up to 3.500. Absolute values greater
  than 3.500 are out of range and are displayed as either *.*** or
  -*.***.

  2.5.3 Memory Backup

  The battery backup provides memory even after the reader is
  turned off. The following information will be saved in memory until
  new reading parameters are set by the user or a new plate is read
  by the instrument. Note: If reading a new plate is aborted before
  it is finished, all the previous plate reading date will remain in
  memory and none of the new data from the new plate is retained.

  1. 10 latest plate reading data for End point protocol

  2. 2 latest sets of Kinetic plate reading data (maximum 30
  series of readings for each Kinetic)

  3. End Point and kinetic protocol data

  4. A set of Checkmark protocol data and the latest set of
  Checkmark read data

  5. 8 wavelength values for filters

  6. Laboratory name

  7. 2 security passwords (Administrator and Common user)

  The first time the instrument is turned on, or after a battery
  replacement, the following default information will hold in
  memory:

  1. Both Administrator and Common user security password are set
  to 00000

  2. Laboratory name is Bio-Rad Laboratories

  3. All wavelength values of the filters are set to — nm

  4. The date and time of the calendar chip are set to 1 day, 1
  month, 00 year, 0 hour and 0 minutes

  5. None of the plate reading data is available

  6. End Point protocol is chosen and the protocol number is set
  to #1

• System Overview

  2-4 100-2414C 03/04

  2.5.4 Reports

  Eight types of reports for End Point protocol can be generated
  by the Model 680 Microplate Reader:

  1. Raw Data Report2. Absorbance report3. Limit Report4. Matrix
  Report5. Cut Off report6. Curve Fit Report7. Concentration Report8.
  Difference report

  2.5.5 RS-232 Interface

  The Model 680 Microplate Reader has a built-in RS232C interface
  (9 pin D-sub connector). This allows external computers to control
  the instrument. When an external computer is in control of the
  microplate reader, the printer and the keypad keys (except
  Start/Stop) are automatically deactivated and the display will
  indicate Remote Mode.

  The reader will remain in the remote control mode until it
  receives RL command from the host computer or until the Start/Stop
  key is pressed.

  RS-232 communication parameters are as follows:

  Signals: TDX: Transmit DataRXD: Receive DataDTR: Data Terminal
  ReadyDSR: Data Set ReadyCTS: Clear to SendRTS: Ready to Send

  Transmission: Baud Rate 9600, Data Width 8, Stop Width 1, Parity
  None

  Data: ASCII (no distinction between upper and lower case)

  Signal Assignment:

  Host Computer9 Pin D-sub

  Model 680 Reader9 Pin D-sub

  Receive Data 2 ———————— 3 Transmit Data

  Transmit Data 3 ———————— 2 Receive Data

  Data Terminal Ready 4 ———————— 6 Data Set
  Ready

  Signal Ground 5 ———————— 5 Signal Ground

  Data Set Ready 6 ———————— 4 Data Terminal
  Ready

  Request to Send 7 ———————— 8 Clear to Send

  Clear to Send 8 ———————— 7 Request to Send

• System Overview

  100-2414C 03/04 2-5

  2.6 Instrument Specifications

  The Model 680 and 680 XR Microplate Reader specifications are as
  follows:

  2.6.1 Features

  Operating Panel: Membrane pad with 4 arrow keys, 7 function keys
  and 11 numeral keys

  Display: LCD, 4 Lines x 20 Alphabet Characters

  Computer Interface: Bi-directional RS-232C serial communication
  port, 9 pin D-sub receptacle

  External Printer Port (only when internal printer option is not
  installed): Centronics interface with ASCIIcharacter code; 25 pin
  D-sub receptacle; 80 characters output per line

  Report Types: Raw, absorbance, matrix, limit, cutoff,
  concentration, curve fit, difference, kinetic

  Applicable Plates: Standard 96 well microtitre plate of
  polystyrene — flat, U, V rigid bottom

  Mixing: 3 speeds (low, medium, high); Programmable time period
  0-999 seconds

  Warm-up Time: 3 minutes

  2.6.2 Optical

  Photometric Methods: Single and dual wavelength

  Photo detectors: 8 silicon diodes for measurement and 1 silicon
  photodiode for reference

  Light Source: Tungsten halogen lamp, 20 Watts with 3000 hours
  average lifetime

  Model 680 Spectral Range: 400 nm to 750 nm

  Model 680 XR Spectral Range: 340 nm to 800 nm

  Interference Filters: Wavelength Tolerance +/- 3 nm; Band Width:
  10 nm typical

  Filter Capacity: 8 filters

  Model 680 Standard Filters: 415, 450, 490, 655 nm

  Model 680 XR Standard Filters: 340, 405, 415, 655 nm

  Optional Filters: 340 through 800 nm (in 5 nm increments)

  Fast Reading Speed: 6 seconds with single wavelength reading; 10
  seconds with dual wavelengthreading

  Standard Reading Speed: 15 seconds with single wavelength
  reading; 30 seconds with dual wave-length reading

  Indication Range: 0.000 to 3.5 OD

  2.6.3 Performance

  Resolution: 0.001 OD

  Accuracy: +/- 1.0% (or 0.010 from 0 to 3.0 OD at 490 nm)

  Linearity: +/- 1.0% (from 0 to 2.0 OD); +/- 2.0% (from 2.0 to
  3.0 OD)

  Reproducibility: 1.0% (or 0.005 from 0 to 2.0 OD); 1.5% (from
  2.0 to 3.0 OD)

  Inter-Channel Variation: 1.5% (or 0.005 from 0 to 3.0 OD)

  Stability and Drift: 0.010 OD at OD=1 with single wavelength
  reading

• System Overview

  2-6 100-2414C 03/04

  2.6.4 Thermal

  Incubator (Optional): Temperature Set Point: 25oC to 45oC (at
  least 5oC above ambient)

  Set Point Accuracy: +/- 0.5oC (set point = 37oC and room
  temperature = 25oC)

  Well-to-Well Uniformity: 0.5oC (set point = 37oC and room
  temperature = 25oC)

  Warm-up Time: 5 minutes (set point = 37oC and room temperature =
  25oC)

  2.6.5 Electrical

  Line Voltage: Universal Input: 100-120 VAC, 200-240 VAC, 50/60
  Hz

  Fuse: T3.15 Amp, 250 Volts, 20mm x 5mm

  Power Consumption: 100 VA maximum

  Battery: Lithium, CR2032, 3 Volts; average lifetime 5 years

  2.6.6 Physical

  Dimensions: 340 x 330 x 152 mm

  Weight: 5.5 kg

  Operating Environment: 5oC to 35oC, 0 to 95% RH, non
  condensing

  Storage Environment: -20oC to 50oC, 0 to 95% RH, non
  condensing

• Installation

  100-2414C 03/04 3-1

  Section 3Installation

  3.1 Introduction

  The Model 680 and 680 XR Microplate Readers are easy to install.
  Prior to installation, verify that all systemcomponents have been
  received.

  3.2 Visual Check

  1. Check this instrument for any signs of damage which may have
  occurred during shipping.

  2. Check the filters: Open the rear cover by pushing the release
  latch on the top. Check that the filters are installed into the
  proper positions per Table 1:

  Table 1 — Filter Installation

  3. Check to insure filters are properly seated (Figure 1).

  Figure 1: Filter Check

  Filter Position Model 680 Model 680XR

  1 415 nm 340 nm

  2 450 nm 405 nm

  3 490 nm 415 nm

  4 655 nm 655 nm

  5 User User

  6 User User

  7 User User

  8 User User

• Installation

  3-2 100-2414C 03/04

  3.3 Installation Procedure

  1. Place the instrument on a clean, sturdy table or bench. It is
  important to keep the instrument in a clean, relatively dust free
  environment to ensure maximum performance.

  2. Connect the power cord to the connection on the back if the
  instrument.

  3. Turn ON the power switch on the rear panel. The LCD will
  display the version number of the on board firmware. After about
  three seconds, the instrument will perform a self test diagnosis
  that takes about fifteen seconds.

  4. When prompted by the Log In screen, enter the password
  (password = 00000). Press Enter.

  5. Allow three minutes for the instrument to warm up prior to
  reading plates.

  3.4 Install Paper Roll (Optional Internal Printer)

  1. Open the rear cover by pushing the release latch on the
  top.

  2. Tear off a small piece of paper to form a diagonal point on
  the end of the paper roll.

  3. Place the paper roll in the holder positioned below the
  printer and orient the roll so that it will feed from the bottom
  (Figure 2).

  Figure 2: Orientation of Paper Roll

  4. While pressing the Paper Feed key, direct the pointed end of
  the paper into the paper feed slot in the underside of the printer
  until the printer grabs the paper and feeds it through the slot in
  the top of the instrument.

• Installation

  100-2414C 03/04 3-3

  3.5 Computer Interface (Optional)

  1. Some customers may utilize the system with a computer and
  microplate software such as Microplate Manager (or Checkmark).

  2. Set up computer as required and install software.

  3. Install serial cable from computer to Model 680 oe 680
  XR.

  4. Turn on instrument, enter password.

  5. Verify communication with instrument using Microplate
  Manager.

  Note: Microplate Manager version 5.2.1 is required for Model
  680XR support.

• System Checkout

  100-2414C 03/04 4-1

  Section 4System Checkout

  4.1 Introduction

  This section provides the system checkout procedure for the
  Model 680 and 680 XR Microplate Reader. Suc-cessful completion of
  the tests in this section represent the Operational Qualification
  of the instrument. A Sys-tem Checkout Report is available in
  Appendix A and B.

  The system checkout procedures for the Model 680 and 680 XR
  Microplate Readers require the use of aremote computer using
  Microplate Manager software.

  The Model 680 and 680 XR Microplate Reader contains an on board
  checkout procedure, Checkmark Valida-tion Program, which requires
  the use of a special Checkmark test plate.

  4.2 Tools and Materials

  Microplate Manager Software, 170-9520

  RS-232 Cable, 931-0184

  Printer

  Digital Temperature Multi-meter with 0.1 mm diameter bead type K
  thermoprobe (minimum +/- 0.5 C temperature meter accuracy)

  96 Well Microtitre Plate, Flat Bottom, 224-0096

  Micro Pipette, 50L

  Pipette Tips

  50 ml beaker

  Acid Yellow 99, Fisher AC19178-100

  Distilled Water

  Tween 20, 170-6531

  Checkmark Reader Performance Check Plate, 170-6940
  (Optional)

• System Checkout

  4-2 100-2414C 03/04

  4.3 Summary of Tests and Specifications

  The following is a summary of the tests and specifications for
  the Model 680 and 680 XR Microplate Readers.

  Table 1: Summary of Tests and Specifications

  Test Parameter Specification

  Firmware Firmware Version Record Version

  Instrument Function Keypad and Display Proper Function

  Internal Printer (optional feature) Proper Function

  Startup and Self Diagnosis Proper Function

  RS-232 Communication Proper Communication

  Mixing Feature Proper Function

  Incubator Set Point 37 C (optional feature) 37 C +/- 0.5 C

  System Checkout Air Read < 0.005 OD

  Accuracy CV < 5%

  Channel-to-Channel CV < 5%

  Stability and Drift < 0.010 OD

• System Checkout

  100-2414C 03/04 4-3

  4.4 System Checkout Procedure

  4.4.1 General

  1. All system checkout procedures in this section will be
  performed with Microplate Manager via PC computer.

  2. The Checkmark Validation Program, if available, may be used
  for system checkout as an alternate method (paragraph 4.5).

  4.4.2 Setup

  1. Setup computer.

  2. Install Microplate Manager.

  3. Install HPK protection key.

  4. Connect RS-232 cable from computer to reader.

  4.4.3 Preparing Yellow Dye Test Plate

  1. System checkout requires the preparation of a yellow dye
  pipetted into a microtitre plate. Pipetting must be of extreme
  accuracy as the Model 680 reader is sensitive enough to detect
  pipetting errors.

  2. Prepare the yellow dye as follows:

  Mix: 2 mg Acid Yellow 9950 ml Distilled Water0.01 ml Tween
  20

  3. Pipette 200 microliters yellow dye into each well of the
  microtitre plate (96 total).

  4. The value of the prepared test plate is 0.424 — 0.444 OD.

  4.4.4 Startup

  1. Turn ON the reader power switch. The LCD will display the
  version number of the on board firmware. Record Firmware
  Version.

  2. After about three seconds, the instrument will perform a self
  test diagnosis that takes about ten sec-onds.

  3. When prompted by the Log In screen (Administrator), enter the
  password 00000. Press Enter.

  4.4.5 Touch Pad/Mixing Function

  1. Open chamber door (leave open for this test).

  2. Press Memory Recall button.

  3. Select Protocol and press Enter.

  4. Select End Point and press Enter.

  5. Select End Point 01 and press Enter twice.

  6. Press the Start button.

  7. Observe that plate carrier vibrates for 3 seconds prior to
  read.

• System Checkout

  4-4 100-2414C 03/04

  4.4.6 Verify Printer (Optional Feature)

  1. After completion of the above End Point 01 test, press the
  Print button.

  2. Select Reports and press Enter.

  3. Verify the printer is functioning properly.

  4.4.7 Start Microplate Manager

  1. Start Microplate Manager.

  2. Select File…Select Reader. Select Model 680 or Model 680 XR
  and click OK.

  3. Select New Endpoint Protocol. The Microplate Manager window
  will appear (Figure 1).

  Figure 1: Microplate Manager — Endpoint Protocol

  4. The instrument will be placed in remote mode. Remote Mode
  will appear on display and touch keys will become
  nonfunctional.

• System Checkout

  100-2414C 03/04 4-5

  4.4.8 Air Read

  1. This test is performed without the use of a microtiter
  plate.

  2. Press Show Template button.

  3. Click the Turn Off Autofill Icon.

  4. Click the Format as Unknowns Icon.

  5. Using the curser, click and drag highlighting all rows (A1 to
  G12).

  6. All wells on the template should be squares filled with 1s
  (see Figure 2).

  Figure 2: Plate Template

  7. Minimize the plate template.

  8. Click on Reports.

  9. Check the Raw Data Report. Click OK. (See Figure 3).

  Figure 3: Raw Data Report Setup

• System Checkout

  4-6 100-2414C 03/04

  10. Check the Reading Parameters Single box.

  11. Select Measurement filter: 490.

  12. Click the Run button.

  13. Plate will take a few moments to read and display the Raw
  Data Report.

  14. Ensure that all values are < 0.005. Reference Figure
  4.

  Figure 4: Raw Data Report

• System Checkout

  100-2414C 03/04 4-7

  4.4.9 Accuracy

  1. This test is performed with the use of the yellow dye test
  plate.

  2. Use the formatted plate template from the Air Read above.

  3. Click on Reports.

  4. Check the Raw Data Report and Unknown Concentrations. Click
  OK. See Figure 5.

  Figure 5: Report Selection

  5. Check the Reading Parameters Single box.

  6. Select Measurement filter: 490.

  7. For Mix Time (sec) enter 3.

  8. Click the Run button.

  9. Ensure the CV is < 5% on the Unknown Concentrations Report
  (Figure 6) and target values are within expected ranges (Mean
  OD).

  Figure 6: Unknown Concentrations Results

• System Checkout

  4-8 100-2414C 03/04

  4.4.10 Channel-to-Channel Variation

  1. This test is performed with the use of the yellow dye test
  plate.

  2. Press Show Template button.

  3. Click the Turn Off Autofill Icon.

  4. Click the Format as Unknowns Icon.

  5. Using the curser, click and drag highlighting rows A1 to A12.
  The number 1 should appear in each box. Repeat highlighting rows B1
  to B12. The number 2 should appear in each box. Repeat for the
  remaining rows C1 to C12, D1 to D 12, etc. All wells on the
  template should appear as shown in Fig-ure 7.

  Figure 7: Channel to Channel Setup

  6. Minimize the plate template.

  7. Use the same format for reports (Raw Data Report and Unknown
  Concentrations), filter (490) and mix time (3).

  8. Click the Run button.

  9. Plate will take a few moments to read and display the Raw
  Data Report and Unknown Concentra-tions Report.

  10. Ensure the CVs are < 5% on the Unknown Concentrations
  Report (Figure and target values are within expected ranges
  (Mean OD).

• System Checkout

  100-2414C 03/04 4-9

  Figure 8: Unknown Concentrations Report

  4.4.11 Stability and Drift

  1. This test uses the same setup as specified in 4.4.11
  Channel-to-Channel Variation above.

  2. Rerun the Channel-to-Channel variation test 3 times allowing
  10 minutes between tests.

  3. Compare the data from the three tests. The average mean from
  each row shall be within 0.010 OD of each other.

• System Checkout

  4-10 100-2414C 03/04

  4.4.12 Verify Incubator Temperature (Optional Feature)

  1. Place checkmark in Incubator On box. Insure set point is 37
  C.

  2. Place a standard 96 well microtitre plate filled with 200
  microliters water in wells A1, A6, A12, D6, D9, H1, H6, and H12 in
  the plate carrier. See Figure 9.

  Figure 9: Designated Temperature Test Points

  3. Allow microtiter plate to warm for 30 minutes.

  4. After 30 minutes, immerse the thermoprobe in the designated
  well filled with the 200 microliters of water without touching any
  portion of the inner well wall. The immersed probe must be in the
  middle of the well and immersed in water. See Figure 10.

  Figure 10: Well Temperature Measurement

  5. Verify that the actual well temperature is 37 C +/- 0.5 C for
  each well (A1, A6, A12, D6, D9, H1, H6, and H12).

• System Checkout

  100-2414C 03/04 4-11

  4.5 Checkmark Validation Program (Optional Method)

  4.5.1 General

  Figure 11: Checkmark Check Plate

  1. The Checkmark program is used with the Checkmark Reader
  Performance Check Plate for validat-ing the performance of the
  Model 680 Microplate Reader. It will test the following
  parameters:

  Default Measurements

  Accuracy

  Precision

  Linearity

  Optional Measurements

  Plate Positioning

  Cross-talk

  Filter Check

  0 OD and Overflow

  IR Spectral Blocking

  2. The Checkmark program resides in the Model 680 Microplate
  Reader programs.

  4.5.2 Procedure

  Entering Checkmark Plate Reference Data

  1. Turn on Model 680 Reader and enter password.

  2. Press Memory Recall button.

  3. Select Protocol and press Enter.

  4. Select Checkmark and press Enter. Press any key if
  instructed.

  5. Press the Edit button.

  6. Select Protocol and press Enter.

• System Checkout

  4-12 100-2414C 03/04

  7. Select Serial/Report and press Enter

  8. Enter serial number of Checkmark plate. Select report type
  Simple or Full. Press Enter.

  9. Select Linearity and press Enter.

  10. Enter appropriate data from Checkmark reference plate data.
  Press enter when complete.

  11. Select Max CV and press Enter.

  12. Enter appropriate data from Checkmark reference plate data.
  Press enter when complete.

  13. Select Filter Check Reference and press Enter.

  14. Enter appropriate data from Checkmark reference plate data.
  Press enter when complete.

  Running the Test

  1. Open chamber door, insert Checkmark Reader Performance Check
  Plate, and close door.

  2. Press Memory Recall button.

  3. Select Protocol and press Enter.

  4. Select Checkmark and press Enter. Press any key if
  instructed.

  5. Press the Start button.

  6. Test will take about 3 minutes to read plate.

• Preventative Maintenance

  100-2414C 03/04 5-1

  Section 5Preventative Maintenance

  5.1 Introduction

  This section provides the preventative maintenance procedure for
  the Model 680 and 680 XR MicroplateReader. Preventative maintenance
  is recommended at least once a year.

  5.2 Tools and Materials

  Small Hand Tools

  Cleaning Materials (Lint Free Cloth, Lens Tissue, Isopropyl
  Alcohol)

  5.3 Preventative Maintenance

  5.3.1 Firmware

  1. Connect the power cord to the connection on the back if the
  instrument.

  2. Turn ON the power switch on the rear panel. The LCD will
  display the version number of the firm-ware.

  3. Record firmware version.

  4. Turn OFF instrument.

  5. Update to latest firmware if applicable (see Section
  7.6).

  5.3.2 Inspection of Instrument

  1. Remove top cover.

  2. Visually inspect for cleanliness of plate carrier. Clean
  plate carrier and rails with lint free cloth and isopropyl
  alcohol.

  Note: Do not use grease on the plate carrier or rails.

  3. Visually inspect for cleanliness of lenses. Clean as required
  with lens tissue and isopropyl alcohol (see Section 5.3.3).

  4. Reassemble instrument.

• Preventative Maintenance

  5-2 100-2414C 03/04

  5.3.3 Optics Cleaning

  1. Remove top cover.

  2. To access top lenses, remove the two screws securing the
  fiber optics to the cover (Figure 5-1).

  Figure 5-1: Remove Screws Securing Fiber Optics

  3. Remove two hex standoffs securing top lens block.

  4. To remove lenses, remove ten securing screws (Figure
  5-2).

  Figure 5-2: Top Lens Block

  5. Clean lenses with lint free cloth and isopropyl alcohol.

  6. Reassemble lenses into holder and remount.

  Note: Check for proper orientation when remounting.

• Preventative Maintenance

  100-2414C 03/04 5-3

  7. To access bottom lenses, remove cover (Figure 5-3).

  Figure 5-3: Bottom Lenses

  8. Clean lenses as required. If removal of bottom lenses is
  required for cleaning, remove plate carrier and analog board (see
  Section 6.3.12).

  9. Remove bottom lens block (Figure 5-4).

  Figure 5-4: Bottom Lens Block Screws

• Preventative Maintenance

  5-4 100-2414C 03/04

  10. Remove lenses from holder to clean (Figure 5-5).

  Figure 5-5: Bottom Lens Holder

  11. Clean with lint free cloth and isopropyl alcohol.

  12. Reassemble instrument. Note proper orientation of bottom
  lens holder upon resaaembly.

  5.3.4 Perform System Checkout

  1. Perform system checkout per Section 4.0 of this Service
  Manual.

• Removal and Replacement

  100-2414D 09/05 6-1

  Section 6Removal and Replacement

  6.1 Introduction

  This section provides the parts removal and replacement
  procedures for the Model 680 and 680 XR MicroplateReader.

  6.2 Tools and Materials

  Standard Hand Tools

  Voltmeter

  6.3 Removal and Replacement Procedures

  6.3.1 Interference Filters

  1. Open the rear cover by pushing the release latch on the
  top.

  2. Rotate the filter wheel until the desired filter is in top
  position. Grasp top of filter frame to and pull up to remove
  filter.

  3. To install filter, insert into desired slot until filter
  locks into place.

  6.3.2 Model 680 Lamp (1681006)

  1. Open the rear cover by pushing the release latch on the
  top.

  Caution: Surface of Cover may be Hot!

  2. Remove the two screws that hold the black plastic cover in
  place (Figure 6-1) and remove cover.

  Figure 6-1: Screws Securing Cover

• Removal and Replacement

  6-2 100-2414D 09/05

  3. Remove the screw which holds the metal cover in place (Figure
  6-2) and remove cover.

  Figure 6-2: Screw Securing Metal Cover (Caution: Hot)

  4. Carefully pull the lamp free and remove (Figure 6-3).

  Figure 6-3: Lamp Removal

  5. Install new lamp in reverse order of the above steps.

• Removal and Replacement

  100-2414D 09/05 6-3

  6.3.3 Model 680 XR Lamp (1681104)

  1. Open the rear cover by pushing the release latch on the
  top.

  Note: Surface of Cover may be Hot! Allow to cool before
  replacing lamp.

  2. Remove the two screws (arrows) that hold the black plastic
  cover in place (Figure 6-4) and remove cover assembly.

  Figure 6-4: Screws Securing Cover

  3. Remove the lamp (Figure 6-5).

  Figure 6-5: Lamp Removal

  4. Install new lamp.

  Note: Do not tough new lamp with fingers. Oil on lamp may reduce
  lamp life.

  5. Reassemble in reverse order of the above steps.

• Removal and Replacement

  6-4 100-2414D 09/05

  6.3.4 Top Cover (9310161)

  1. Remove the six screws from the underside of the instrument
  (Figure 6-6).

  Figure 6-6: Remove Six Screws from Underside of Instrument

  2. Remove the top cover to left side of the instrument (Figure
  6-7).

  Figure 6-7: Remove Top Cover to Left Side of Instrument

  3. Install cover in reverse order of the above steps.

• Removal and Replacement

  100-2414D 09/05 6-5

  6.3.5 Printer Assembly (9310160)

  1. Remove top cover.

  2. Cut the cable tie securing the cable and remove cable from
  the circuit board (Figure 6-8).

  Figure 6-8: Cut Cable Tie and Remove Cable

  3. Remove three screws securing printer assembly and remove
  assembly (Figure 6-9).

  Figure 6-9: Three Screws Securing Printer Assembly

  4. Prior to installing printer assembly, set the jumpers on the
  printer control board as follows:

• Removal and Replacement

  6-6 100-2414D 09/05

  5. Check printer head type on flexible cable (Figure 6-10).

  Figure 6-10: Location of Head Type

  6. Configure the JP4 jumpers in accordance with the Figure
  6-11.

  Figure 6-11: JP4 Jumper Settings

  7. Install new printer in reverse order of the above steps.

• Removal and Replacement

  100-2414D 09/05 6-7

  6.3.6 Keypad Membrane (9310177, 9310277, 9310229)

  1. Remove top cover.

  2. Cut cable tie and disconnect three cables from keypad board
  (Figure 6-12).

  Figure 6-12: Keypad Board

  3. Remove three screws and lift out keypad board (Figure
  6-13).

  Figure 6-13: Screws Securing Keypad Board

  4. Peel off keypad membrane (Figure 6-14).

  Figure 6-14: Peel Off Keypad Membrane

  5. Install new keypad membrane in reverse order of the above
  steps.

• Removal and Replacement

  6-8 100-2414D 09/05

  6.3.7 Inlet Assembly (9310167)

  1. Remove top cover.

  2. Disconnect the terminals from board (Figure 6-15).

  Figure 6-15: Disconnect Terminals

  3. Remove nuts from interface connectors and remove (Figure
  6-16).

  Figure 6-16: Remove Nuts from Connectors

  4. Remove four screws securing the fan (Figure 6-17).

  Figure 6-17: Fan Mounting Screws

  5. Remove inlet assembly.

  6. Install new inlet assembly in reverse order of the above
  steps.

• Removal and Replacement

  100-2414D 09/05 6-9

  6.3.8 Plate Carrier Assembly and Analog Board

  Note: Check the model and serial number for the correct
  replacement board.

  Table 6-1: Analog Board Part Numbers

  1. Remove top cover.

  2. Loosen the two screws which secure the fiber and pull out
  fiber (Figure 6-18).

  Figure 6-18: Fiber Mounting

  3. Remove the nine mounting screws (Figure 6-19).

  Figure 6-19: Plate Carrier Mounting Screws

  Model 680 Model 680 XR

  Analog Board 9310182 9310289

• Removal and Replacement

  6-10 100-2414D 09/05

  4. Lift the plate carrier assembly, disconnect the cables and
  remove assembly (Figure 6-20).

  Figure 6-20: Disconnect Cables

  5. Remove two screws securing the cover over the analog board
  (Figure 6-21).

  Figure 6-21: Analog Board Mounted to Plate Carrier

• Removal and Replacement

  100-2414D 09/05 6-11

  6. Carefully remove fiber from mounting clips (Figure 6-22).

  Figure 6-22: Clips Securing Fiber

  7. Remove four screws securing analog board and remove (Figure
  6-23).

  Figure 6-23: Analog Board Mounting Screws

  8. Install new plate carrier assembly or analog board in reverse
  order of the above steps.

• Removal and Replacement

  6-12 100-2414D 09/05

  6.3.9 CPU Board

  Note: Check the model and serial number for the correct
  replacement board.

  Table 6-1: CPU Board Part Numbers

  1. Open top cover.

  2. Remove plate carrier assembly (see 6.3.8).

  3. Remove all cables to CPU board.

  4. Remove four mounting screws and remove board (Figure
  6-24).

  Figure 6-24: CPU Board Mounting

  5. Install new CPU board in reverse order of the above
  steps.

  6. Set the jumper on the CPU Board (#281) per the following
  table:

  Model 680Old LCD

  Model 680New LCD

  Model 680Backlit LCD

  Model 680 XR

  Serial Number 10001 to 10735 10736 to 13850 13851 and higher
  All

  CPU Board 9310181 9310225 9310353 9310278

  Table 6-2: JP2 Jumper Settings for CPU Board

  Instrument Configuration

  Jumper Jumper Placement

  Without Incubator JP2 Jump

  With Incubator JP2 No Jumper (Open)

• Removal and Replacement

  100-2414D 09/05 6-13

  6.3.10 Incubator Control Board (9310180)

  1. Open top cover.

  2. Remove plate carrier assembly (see 6.3.8).

  3. Remove all cables to incubator control board.

  4. Remove four mounting screws and remove board (Figure
  6-25).

  Figure 6-25: Incubator Control Board Mounting

  5. Install new incubator control board in reverse order of the
  above steps.

  6. Perform incubator electronics adjustment as follows.
  Disconnect cable from plate carrier to CN52.

  7. Connect cable into Incubation Adjustment Tool, 9310183,
  107.79 ohm connector on tool.

  8. Turn on instrument.

  9. Measure voltage between TP5 and TP0 (see Figure 6-26).

• Removal and Replacement

  6-14 100-2414D 09/05

  Figure 6-26: Incubator Board Adjustments

  10. Adjust trim pot VR1 until the voltage measures 0.000V +/-
  0.001V.

  11. Connect cable into Incubation Adjustment Tool, 116.47 ohm
  connector on tool.

  12. Measure voltage between TP5 and TP0.

  13. Adjust trim pot VR1 until the voltage measures 5.528V +/-
  0.006V.

  14. If voltage is out of range, repeat steps 3 through 9.

• Removal and Replacement

  100-2414D 09/05 6-15

  6.3.11 Model 680 XR Lamp Control PCB (9310276)

  1. Open top cover.

  2. Remove ground screw connection and four mounting screws and
  lift up the filter wheel/lamp assem-bly (Figure 6-27).

  Figure 6-27: Lamp Control PCB is Located Under Lamp Assy

  3. For ease of disassembly, turn over assembly. To access lamp
  control board, remove the five screws securing the lamp PCB cover
  (Figure 6-28).

  Figure 6-28: Bottom View — Lamp PCB Cover Screws

  4. Remove cover.

  Note: Heat sink will be removed as a result of removing the two
  side screws.

  Ground Screw

  Two Screws Backside (Not Shown)

• Removal and Replacement

  6-16 100-2414D 09/05

  5. Remove the two remaining screws securing the PCB (Figure
  6-29).

  Figure 6-29: Lamp Control PCB

  6. Remove three cables to lamp control PCB and lift out.

  7. Install new lamp control PCB by securing with two mounting
  screws and attaching the three cables.

  8. Install the cover and heat sink. For ease of assembly,
  position the heat sink in place prior to placing over lamp control
  PCB (Figure 6-30).

  Figure 6-30: Position Heat Sink

  9. Install the two screws heat sink side first, then the two
  remaining screws securing the cover.

  10. Reassemble in reverse order of the above steps.

• Removal and Replacement

  100-2414D 09/05 6-17

  6.3.12 Power Supply, 12 Volt (9310178)

  1. Open top cover.

  2. Remove plate carrier assembly (see 6.3.8).

  3. Remove four screws securing lamp housing and remove (Figure
  6-31).

  Figure 6-31: Lamp Housing Mounting

  4. Remove all cables to 12 volt power supply.

  5. Remove four mounting screws and remove power supply (Figure
  6-32).

  Figure 6-32: 12 Volt Power Supply Mounting

  6. Install new 12 volt power supply in reverse order of the
  above steps.

• Removal and Replacement

  6-18 100-2414D 09/05

  6.3.13 Power Supply, 24 Volt (9310179)

  1. Open top cover.

  2. Remove plate carrier assembly (see 6.3.8).

  3. Remove four screws securing lamp housing and remove (Figure
  6-33).

  4. Remove all cables to 24 volt power supply.

  5. Remove four mounting screws and remove power supply (Figure
  6-33).

  Figure 6-33: 24 Volt Power Supply Mounting

  6. Install new 24 volt power supply in reverse order of the
  above steps.

• Removal and Replacement

  100-2414D 09/05 6-19

  6.3.14 Photo Sensor — Plate Carrier/Analog Board (9310174)

  1. Remove top cover.

  2. Open the clip securing the optical fiber assembly. Remove the
  three screws securing the cover and remove (Figure 6-34).

  Figure 6-34: Remove Clip and Mounting Screws

  3. Remove the screw securing the photo sensor and remove (Figure
  6-35).

  Figure 6-35: Removing the Photo Sensor

  4. Install new photo sensor in reverse order of the above
  steps.

  5. Check plate positioning per Section 7.5.

• Removal and Replacement

  6-20 100-2414D 09/05

  6.3.15 Photo Sensor — Filter Wheel (9310174)

  1. Remove top cover.

  2. Remove cable from photo sensor. Remove screw securing photo
  sensor and remove (Figure 6-36).

  Figure 6-36: Photo Sensor Removal

  3. Install new photo sensor in reverse order of the above
  steps.

• Removal and Replacement

  100-2414D 09/05 6-21

  6.3.16 Model 680 Light Balancing Filter (9310173)

  1. Remove top cover.

  2. Remove plastic cover and metal cover from atop lamp.

  3. Remove two screws securing lamp housing (Figure 6-37).

  Figure 6-37: Lamp Housing

  4. Remove two screws securing the light balancing filter (Figure
  6-38).

  Figure 6-38: Light Balancing Filter

  5. Install new light balancing filter in reverse order of the
  above steps.

• Removal and Replacement

  6-22 100-2414D 09/05

  6.3.17 Model 680 XR Light Balancing Filter (9310275)

  1. Remove top cover.

  2. Remove two screws (arrows) securing lamp housing assembly
  (Figure 6-39).

  Figure 6-39: Lamp Housing

  3. Remove two recessed screws securing the light balancing
  filter (Figure 6-40).

  Figure 6-40: Light Balancing Filter

  4. Install new light balancing filter in reverse order of the
  above steps.

• Removal and Replacement

  100-2414D 09/05 6-23

  6.3.18 Plate Carrier Motor (9310172)

  1. Remove top cover.

  2. Remove the three cover screws and lift away (Figure
  6-41).

  Figure 6-41: Remove Inner Cover

  3. Remove the two screws from the rail cover and remove cover
  (Figure 6-42).

  Figure 6-42: Cover Screws

• Removal and Replacement

  6-24 100-2414D 09/05

  4. Loosen the screw to the belt and remove belt Figure
  6-43).

  Figure 6-43: Loosen Belt Adjustment Screw

  5. Remove two screws securing motor (Figure 6-44). Remove and
  unplug motor.

  Figure 6-44: Motor Mounting Screws

  6. Install new motor in reverse order of the above steps.

• Removal and Replacement

  100-2414D 09/05 6-25

  6.3.19 Optical Fiber Assembly (9310169)

  1. Open top cover.

  2. Remove plate carrier assembly (see 6.3.8).

  3. Open the clip securing the optical fiber assembly. Remove the
  three screws securing the cover and remove (Figure 6-45).

  Figure 6-45: Removing Optical Fiber Assembly

  4. Remove two screws securing the cover over the analog
  board

  5. Carefully remove fiber from mounting clips (Figure 6-46).

  Figure 6-46: Remove Cover Screws

  6. Install new optical fiber assembly in reverse order of the
  above steps.

• Removal and Replacement

  6-26 100-2414D 09/05

  7. When re-installing fiber into filter wheel housing, ensure
  that end is protruding 2 mm from plate (Fig-ure 6-47).

  Figure 6-47: Fiber Installation

• Removal and Replacement

  100-2414D 09/05 6-27

  6.3.20 Filter Wheel (9310170) & Filter Wheel Motor
  (9310171)

  1. Open top cover.

  2. Remove plate carrier assembly (see 6.3.8).

  3. Cut cable tie and remove two screws securing the filter wheel
  assembly (Figure 6-48).

  Figure 6-48: Filter Wheel Assembly Mounting

  4. Unplug cable from motor to analog board.

  5. Remove two screws securing filter wheel to motor (Figure
  6-49).

  Figure 6-49: Filter Wheel Mounting

  6. Remove two screws securing motor to mounting bracket.

  7. Install new filter wheel or motor in reverse order of the
  above steps.

• Removal and Replacement

  6-28 100-2414D 09/05

  6.3.21 Fan (9310168)

  1. Open top cover.

  2. Remove plate carrier assembly (see 6.3.8).

  3. Remove 4 screws and move lamp assembly in order to facilitate
  unpluging of fan cable (Figure 6-50).

  Figure 6-50: Lamp Assembly Mounting

  4. Remove four mounting screws and remove fan (Figure 6-51).

  Figure 6-51: Fan Mounting

  5. Install new fan in reverse order of the above steps.

• Removal and Replacement

  100-2414D 09/05 6-29

  6.3.22 Lithium Battery (9006020)

  1. Open top cover.

  2. Remove plate carrier assembly (see 6.3.8).

  3. Replace the lithium battery located on the CPU board (Figure
  6-52).

  Figure 6-52: Lithium Battery

  4. Install new lithium battery in reverse order of the above
  steps.

• Adjustments, Calibration and Checks

  100-2414E 02/06 7-1

  Section 7Adjustments, Calibration and Checks

  7.1 Introduction

  This section provides the adjustments, calibration and checks
  applicable to the Model 680 and Model 680 XRMicroplate Readers.

  7.2 Tools and Materials

  Standard Hand Tools

  Voltmeter

  9310183 Incubator Adjustment Tool

  9310184 RS-232C Cable

  1002446 Firmware CD

  7.3 Electronics Adjustment

  7.3.1 Setting of Jumper on the CPU Board (#281)

  1. Open the top cover and remove plate carrier assembly to
  access CPU Board (#281).

  2. Locate JP2 (Figure 7-1).

  Figure 7-1: Jumper JP2

  3. Set the jumper on the CPU Board (#281) per the following
  table:

  Table 7-1: JP2 Jumper Settings for CPU Board

  Instrument Configuration

  Jumper Jumper Placement

  Without Incubator JP2 Jump

  With Incubator JP2 No Jumper (Open)

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  7.3.2 Setting of the Jumpers on the Printer Control Board
  (#284)

  1. Open the top cover to access Printer Control Board
  (#284).

  2. Check printer head type on flexible cable (Figure 7-2).

  Figure 7-2: Location of Head Type

  3. Configure the jumpers to JP4 in accordance with the Figure
  7-3.

  Figure 7-3: JP4 Jumper Settings

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  7.3.3 Incubator Electronics Adjustment (Board #284)

  Note: This procedure is used to set the proper temperature set
  points for the incubator.

  1. Open the top cover and remove plate carrier assembly to
  access Incubator board (#284).

  2. Disconnect cable from plate carrier to CN52.

  3. Connect cable into Incubation Adjustment Tool, 9310183,
  107.79 ohm connector on tool.

  4. Turn on instrument.

  5. Measure voltage between TP5 and TP0 (Figure 7-4).

  Figure 7-4: Incubator Board Adjustments

  6. Adjust trim pot VR1 until the voltage measures 0.000V +/-
  0.001V.

  7. Connect cable into Incubation Adjustment Tool, 116.47 ohm
  connector on tool.

  8. Measure voltage between TP5 and TP0.

  9. Adjust trim pot VR1 until the voltage measures 5.528V +/-
  0.006V.

  10. If voltage is out of range, repeat steps 3 through 9.

• Adjustments, Calibration and Checks

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  7.4 Adjusting Plate Carrier Belt Tension

  1. This procedure is used to adjust the belt tension of the
  plate carrier.

  2. Open the top cover.

  3. Remove fiber optics cover (Figure 7-5)

  Figure 7-5: Remove Fiber Optics Cover

  4. Remove two screws securing the belt cover as shown in Figure
  7-6.

  Figure 7-6: Remove Belt Cover

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  5. Loosen the screw and adjust the belt tension. Tension should
  be firm but not overly tight.

  Figure 7-7: Belt Tension Adjustment

• Adjustments, Calibration and Checks

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  7.5 Alignment of Plate Carrier

  7.5.1 Checking Plate Alignment

  1. This procedure is used to check positioning and alignment of
  the plate carrier.

  2. Open the top cover.

  3. Install Checkmark Plate into reader. Make sure plate is
  properly seated.

  4. Apply clear tape over small holes in columns 1 and 12 (Figure
  7-8).

  Figure 7-8: Placement of Tape

  5. Use QC software to move plate beneath optics path per Section
  7.7.3.

  6. Check that the light is centered about the holes in the
  Checkmark Plate in columns 1 and 12 (Figure 7-9).

  Figure 7-9: Checking Plate Position at Columns 1 & 12

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  7.5.2 Adjusting Plate Alignment — Y Direction

  1. This procedure is used to adjust the Y direction (top/bottom)
  position of the plate carrier by reposi-tioning of the rails.

  2. Open the top cover.

  3. Loosen the 12 screws shown in Figure 7-10 and adjust the
  rails as required.

  Figure 7-10: Y Direction Adjustment — Reposition Rails

  7.5.3 Adjusting Plate Alignment — X Direction

  1. This procedure is used to adjust the X direction (left/right)
  position of the plate carrier by repositioning the photo
  sensor.

  2. Open the top cover.

  3. Loosen the screws shown in Figure 7-11 and reposition the
  photo sensor as required.

  Figure 7-11: X Direction Adjustment — Reposition Sensor

• Adjustments, Calibration and Checks

  7-8 100-2414E 02/06

  7.6 Firmware

  7.6.1 General

  1. Downloading firmware is achieved through a PC (Windows 95,
  NT, 2000, XP) and an RS-232 cable.

  2. The communication requirements are as follows:

  RS232CPort: COM1Baud Rate: 9600, 57600Data: 8 bit 1 stop

  3. The downloading program and additional files are required for
  downloading:

  m680_up.exe mscomctl.ocxcomdlg32.ocx msvbvm60.dllmscomm32.ocx
  asycfilt.dllcomcat.dll mci32.ocxoleaut32.dll
  olepro32.dllvb6stkit.dll

  4. The current firmware files are:

  M680_V171OL.txt for Original Lens models (SN 10001 through
  10735)M680_V171NL.txt for New LCD models (SN 10736 through
  13850)M680_V171.txt for Backlit LCD models (SN 13851 and higher)
  M680_V171.txt for Chinese version instrumentsM680XR_V163-XR.txt
  (for Model 680XR readers)

  5. When updating firmware versions 1.60 and earlier with
  m680_up.exe, plate reading results, security password, Checkmark
  reference values, Checkmark reading results and laboratory name
  will be lost. Protocol settings (except for #5-8) and filter
  wavelengths will be retrieved and restored in the update process.
  Date and time will need to be reset.

  6. When updating firmware versions 1.61 and later with
  m680_up.exe, all data and settings except for security password,
  Checkmark reading results, Protocol settings (except for #5-8) and
  Plate Reading results (#5-8) be retrieved and restored in the
  update process. Date and time will need to be reset.

• Adjustments, Calibration and Checks

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  7.6.2 Firmware HistoryTable 7-2: Firmware History

  Instrument Style

  Firmware Version

  History

  Compatibility

  Original LCD

  New LCD

  Backlit LCD

  Original LCDSerial Numbers 10001 — 10735

  1.59 Initial release (compatible with serial num-bers 10001
  through 10735)

  Yes No No

  1.60 Minor corrections (compatible with serial numbers 10001
  through 10735)

  Yes No No

  1.62 Added TSE protocols (compatible with serial numbers 10001
  through 10735)

  Yes No No

  1.63 Changed TSE validation criteria (compatible with serial
  numbers 10001 through 10735)

  Yes No No

  1.71OL Added functionality for ESC/P printer. Added protocols
  for TSE Sheep/Goat and Rabies (compatible with serial numbers 10001
  through 10735)

  Yes No No

  New LCDSerial Numbers 10736 — 13850

  1.60NL Released for new LCD display (SN 10736 through 13850).
  Minor corrections.

  No Yes No

  1.60cNL Released for Chinese version readers (SN 11201 through
  13850).

  No Yes No

  1.62NL Added TSE protocols (compatible with serial numbers 10736
  through 13850)

  No Yes No

  1.63NL Changed TSE validation criteria (compatible with serial
  numbers 10736 through 13850)

  No Yes No

  1.71NL Added functionality for ESC/P printer. Added protocols
  for TSE Sheep/Goat and Rabies (compatible with serial numbers 10736
  through 13850)

  No Yes No

  Model 680XR 1.63XR For Model XR functionality only No Yes No

  Backlit LCDSerial Numbers

  13851 and higher

  1.70 Released for backlit LCD and integration of English and
  Chinese version firmware. Added functionality for ESC/P printer
  (effec-tivity serial number 13851 and higher).

  No No Yes

  1.71 Added protocols for TSE Sheep/Goat and Rabies (effectivity
  serial number 13851 and higher).

  No No Yes

• Adjustments, Calibration and Checks

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  7.6.3 Integration of English and Chinese Firmware Versions

  Note: With firmware version 1.70, the English and Chinese
  versions have been integrated. A language choice has been added
  with this version.

  1. To enter the Select Language screen, press the 0 key and 4
  key and turn ON instrument power. Hold for approximately 5 seconds
  and the Select Language screen will appear.

  2. Use Up and Down arrow keys to select language choice. Press
  Enter.

  3. Reader resets automatically and restarts with the selected
  language.

  Note: If the language selected is different, the reader will
  clear all memory and restore default values.

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  7.6.4 Downloading Firmware

  1. Load the m680_up.exe program. The screen shown in Figure 7-12
  will appear.

  Figure 7-12: Microplate Reader Model 680 Update Screen

  2. Click on Model 680 Update and the following screen will
  appear:

  Figure 7-13: Click Yes after Log-in

  3. Connect a RS-232C cable between Model 680 and PC COM1
  port.

  4. Turn ON Model 680 reader and input password for log in
  (default 00000). Press enter.

  5. When complete, press Yes button on screen.

• Adjustments, Calibration and Checks

  7-12 100-2414E 02/06

  6. The following screen will appear:

  Figure 7-14: Confirmation Screen

  7. Press Yes to update to new version. If the instrument is at
  the latest version, the screen will indicate no update is
  required.

  8. The screen shown in Figure 7-15 will indicate the status as
  existing data and settings are retrieved from the Model 680
  reader.

  Figure 7-15: Retrieving Data Screen

• Adjustments, Calibration and Checks

  100-2414E 02/06 7-13

  9. When data retrieving is complete, the following screen will
  appear:

  Figure 7-16: Firmware Update Screen

  10. The following steps are also indicated on the screen shown
  in Figure 7-16

  11. Turn OFF the Model 680 Reader.

  12. While pushing both Main and 0 key on the keypad, turn on
  reader power switch. After about 10 seconds, the following screen
  on LCD appears:

  Firmware updateAre you sure?Yes: Start KeyNo: 7 Key

  13. Push Start/Stop key switch on the Model 680 reader to begin
  reader firmware erasing.

  14. After the erasing finishes, the LCD screen RS Receive will
  appear.

  15. Click the Start button on PC screen. The firmware will begin
  uploading and bar graph at the bottom of the screen will display
  status. This will take approximately 7 minutes.

  16. When the firmware update operation is complete, the LCD on
  the reader will displayed Finished and the restore previous data
  screen will appear (Figure 7-17).

• Adjustments, Calibration and Checks

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  Figure 7-17: Restore Previous Data Screen

  17. Turn OFF reader power switch then turn ON. A Battery Low
  error message will occur. This is nor-mal; press Enter.

  18. At the Calendar settings screen, enter Date and Time and
  press Enter.

  19. Enter password and press Enter.

  20. After completion of log-in press the Yes button on the PC
  screen.

  21. The restoring data screen will appear (Figure 7-18).

  Figure 7-18: Storing Data Screen

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  22. When the update is complete, the firmware complete message
  will appear (Figure 7-19).

  Figure 7-19: Firmware Update Complete

  23. Click OK to continue.

  24. A password change reminder screen will appear (Figure
  7-20).

  Figure 7-20: Change Password Reminder Screen

  25. Follow on screen instructions to change password or select
  Exit to quit.

• Adjustments, Calibration and Checks

  7-16 100-2414E 02/06

  7.7 QC Software

  Note: There are 4 modes in this program; Measure Mode, Monitor
  Mode, Extension Mode and Incubator. The instructions in this
  section apply to the Monitor Mode which allows step-by-step
  positioning of the plate carrier.

  7.7.1 Entering QC Software

  1. While pressing the 0 and Upper key buttons, turn ON power
  switch. Continue to hold down but-tons until QC Software screen
  appears.

  2. Select YES by pressing the 1 key.

  3. Input the password 00000 and press Enter.

  4. Press the Dot key . to enter the QC Mode menu (Figure
  7-13).

  Figure 7-21: QC Mode

  7.7.2 Monitor Mode

  1. Select Monitor Mode from the menu by pressing 2 (Figure
  7-14).

  Figure 7-22: Monitor Mode

  Definitions:MON = AD Value (voltage conversion)DEC = AD Value
  (decimal)CH = Row PositionPLT = Plate Column PositionFL = Filter
  NumberGain = Gain A, B

  Commands:Main = Back to QC Mode Screen

  Change of Gain A

  Change of Gain B

  Change of Plate Position

  Change of Filter Position

  . (Dot) = Change of Channel

  QC 1: Measure Mode2: Monitor Mode3: Extension Mode4:
  Incubator

  MON: VDEC:CH: A PLT: 12FL: 1 Gain=1.1.1.0

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  7.7.3 Plate Positioning

  1. To use the QC software to check for plate positioning, enter
  the QC Mode and press 2 for Monitor Mode.

  2. The reader will initialize the plate carrier and place column
  12 in the optics path.

  3. Press the down arrow key to change filter position for
  desired filter position.

  4. Press the up arrow key to step the plate through column
  positions 11 to 1.

  7.8 External Printer Setup

  Note: The Model 680 Reader with firmware version 1.70 added
  support for ESC/P external printer mode. Previous versions of
  firmware support only ASCII print mode.

  1. To change the external printer print mode, perform the
  following steps:

  a. Select Edit from the top screen menub. Select Printerc.
  Select desired print mode: ASCII or ESC/Pd. Press Enter

  2. Connect printer and check print application by performing any
  run.

• Troubleshooting

  100-2414E 02/06 8-1

  Section 8Troubleshooting

  8.1 About this Section

  This section of the manual provides information on
  troubleshooting the Model 680 and 680 XR MicroplateReader. The
  information in this section will help diagnose the common operating
  difficulties and componentfailures. If additional assistance in
  resolving a problem is required, please contact the Technical
  ServiceDepartment at 1-800-424-6723.

  8.2 Troubleshooting

  The following troubleshooting tables are intended as a guide to
  assist in diagnosing some common problems.

  Table 8-1: General Problems

  Problem Possible Cause Solution

  Will not communicate with com-puter

  Improper COM Port selected Check that COM1 port is used.

  Improper serial cable Check for proper serial cable .

  CPU board problem Replace CPU board.

  Blank LCD upon Power Up Fuse Blown Check Fuse. If bad,
  replace

  Cable connection Check display cable and reseat.

  Bad LCD Replace LCD.

  Memory Error Bad CPU Board Replace CPU Board.

  Calendar Error Data error Reset time and date. Reboot.

  Bad CPU Board Replace CPU Board.

  Printer Error Printer malfunction Replace printer.

  Will not print to external printer Check printer setup Section
  7.8 (ESC/P requires firmware ver-sion 1.70 or later)

  Battery Error Battery dead Replace battery.

  Old version firmware Download new firmware.

  LCD Hardware Error LCD malfunction Replace LCD.

  A/D Conversion Error Loose cable connection Check cables to CPU
  board and Analog Board

  Bad A/D convertor Replace Analog Board.

  Grinding Sound or Plate Error Carrier binding Check for source
  of binding. Clean and lubricate plate carrier rods as
  necessary.

• Troubleshooting

  8-2 100-2414E 02/06

  Table 8-2: Error Codes

  Problem Possible Cause Solution

  Code 8071- Invalid Command Received data error Resend
  command

  Code 8072 — Parameter Out of range

  Received command parameter is out of range

  Resend command and parame-ter

  Code 8073 — Not in Remote Mode

  Instrument is not in Remote Mode

  Send the AQ command to acquire Remote Control Mode

  Code 8074 — Device Busy Instrument is busy Wait until ready

  Code 8075 — Filter Disk Jammed CPU board bad Replace CPU
  board

  Motor bad Replace motor

  Photo interrupter for Filter disk damaged

  Replace photo interrupter

  Filter disk damage Replace the filter disk

  Code 8076 — Carrier Jammed After Reading

  Plate is not properly seated Reseat plate

  Interference or blockage Check for Interference or block-age

  Code 8077 — Light Bulb Error Lamp defective Replace lamp

  Lamp out of alignment Check that lamp is properly seated

  Optical fiber damaged Replace optical fiber

  Bad analog board Replace Analog Board

  Code 8082 — Filter Wheel Filter wheel jammed Check filter
  wheel

  Code 8083 — Incubator Error Thermo sensor damaged Replace plate
  carrier

  Incubation control board bad Replace Incubation control
  board

  Code 8084 — Carrier Home Sen-sor Closed Status Error

  Photo interrupter for plate carrier damaged

  Replace photo interrupter

  CPU board bad Replace CPU board

  Code 8085 — Carrier Home Sen-sor Open Status Error

  Plate is not properly seated Reseat plate

  Interference or blockage Check for Interference or block-age

  Photo interrupter for plate carrier damaged

  Replace photo interrupter

  CPU board bad Replace CPU board

  Motor bad Replace motor

• Troubleshooting

  100-2414E 02/06 8-3

  Code 8086 — Carrier Jammed Before Reading

  Plate is not properly seated Reseat plate

  CPU board bad Replace CPU board

  Motor bad Replace motor

  Code 8087 — Plate Carrier Posi-tion

  Plate carrier jammed after read-ing

  Check plate carrier for obstruc-tion.

  Code 8088 — Light Level Error Between Channel

  Dirty Lenses Clean lenses

  Optical fiber damaged Replace optical fiber

  Bad analog board Replace Analog Board

  Code 8089 — Filter Missing Filter missing from filter wheel
  allowing light to exceed accept-able range

  Check filter wheel and install filter

  Code 8090 — Security Code in Error

  Security code did not match Remove security lock

  Code 8091 — RS-232C Error Data communication problem Check
  RS-232 cable

  Code 8092 — A/D Conversion Error

  Bad A/D convertor Replace Analog Board

  Code 8093 — Stop Key Pressed Stop key pressed during
  mea-surement

  User error. Reset command

  Table 8-2: Error Codes

  Problem Possible Cause Solution

• Troubleshooting

  8-4 100-2414E 02/06

  Table 8-3: Firmware Downloading

  Problem Possible Cause Solution

  Checksum Error Corrupted Firmware File Repeat using uncorrupted
  file

  Communication Time Out RS-232 not connected Ensure RS-232 is
  properly con-nected.

  Wrong button clicked Repeat download procedure

  RS-232C Error Parity, Overrun or Framing errors occurred

  Ensure RS-232 is properly con-nected. Repeat download
  proce-dure

  Verify Error Communicated data and written data is different

  Repeat download procedure (make sure instrument power is
  cycled)

  Flash Write Error Flash memory could not be writ-ten

  Repeat download procedure (make sure instrument power is
  cycled)

  Flash Erase Error Flash memory could not be erased

  Repeat download procedure (cycle power; first screen to appear
  is downloading screen)

  Erase Check Error No erasing memory exists Repeat download
  procedure (make sure instrument power is cycled)

  Battery Error Occurs after firmware download Normal. Press
  Enter. Reset date, time and filter data.

• Troubleshooting

  100-2414E 02/06 8-5

  Table 8-4: Checkmark Plate Failures

  Problem Possible Cause Solution

  Fails Accuracy, Linearity or Precision

  Erratic or non-uniform well-to-well OD values

  Check for cleanliness of optics and lenses. Clean as
  required.

  Check lamp. Reseat or replace as necessary.

  Check filter. Reseat or replace as necessary.

  Replace fiber optics assembly

  Replace analog board

  Replace CPU board

  Fails Plate Positioning Plate binding Check for proper movement
  of plate.

  Belt tension too tight or too loose Check belt tension and
  tighten/loosen as necessary per Section 7.4

  Plate carrier out of alignment Check plate alignment and
  cor-rect as necessary per Section 7.5

  Failed Cross Talk Wells A2, C2, E2, G2, B3, D3, F3, and H3 are
  exceeding 0.003 OD due to some light path blockage.

  Check for cleanliness of optics and lenses. Clean as
  required.

  Check lamp. Reseat or replace as necessary.

  Check filter. Reseat or replace as necessary.

  Check for proper movement of plate.

  Check belt tension and tighten/loosen if necessary per Section
  7.4

  Check plate alignment and cor-rect as necessary per Section
  7.5

  Replace fiber optic assembly

  Fails Filter Check Filter deterioration Replace filter

  Fails 0 OD/Overflow Not detecting infinite absorbance Check
  plate alignment and cor-rect as necessary per Section 7.5

  Replace analog board

  Fails IR Blocking Detected IR light passing through plate; not
  blocked by filters

  Check filter for damage or deteri-ation. Replace as
  necessary.

• Parts Information

  100-2414E 02/06 9-1

  Section 9Parts Information

  9.1 About this Section

  This section of the manual provides information on the field
  replaceable parts and service tools for the Model680 and 680 XR
  Microplate Readers. Information on parts and tools ordering is also
  included in this section.

  9.2 Parts Orders

  9.2.1 US Domestic Operations

  Parts may be ordered through our Service Parts department. You
  can reach the Service Parts department during Pacific time regular
  office hours by calling 1-510-741-5038 or 5058. You can help to
  make processing your order more efficient by having the following
  information before you call:

  The part number(s) or catalog number(s) of the parts you want to
  order

  Shipment information

  Your account or inventory location number

  9.2.2 International Subsidiaries

  The procedures for ordering parts vary from region to region
  outside the United States. Please contact your Bio-Rad Export Sales
  Representative through your regular sales and service channels.

  9.3 Returning Defective Parts

  9.3.1 US Domestic Operations

  Defective Assemblies returned for evaluation and repair are an
  important part of the logistics and inventory control process.
  Returned parts and assemblies are refurbished when possible, tested
  and returned to ser-vice stock. Maintaining the effectiveness and
  efficiency of this process enables us to deliver the high standard
  of service that our customers expect, at a reasonable cost that
  allows us to remain competitive.

  You are an integral part of our inventory process. When you
  return parts or assemblies to Bio-Rad to be eval-uated and
  repaired, please attach a tag to each assembly. The tag should
  contain the following information:

  Model and serial number of the unit which the part was
  removed

  The part number or catalog number of the part being returned

  The date of removal

  A description of the problem or symptoms exhibited

  Your name and other identification information

  This information will help our service centers to make repairs
  efficiently. When you receive replacement parts,sa